中文摘要：

背景：目前在原代细胞培养领域内,对耳蜗螺旋神经节细胞培养条件的报道各有差异,个别方法重复性较差,不利于实际应用。目的：原代培养并鉴定新生C57小鼠螺旋神经节细胞。方法：显微解剖分离新生C57小鼠蜗轴组织,经胰酶消化＋差速贴壁＋化学药物相结合方法培养;倒置相差显微镜及苏木精-伊红染色观察细胞生长状态,免疫组织化学染色鉴别细胞来源。结果与结论：蜗轴组织细胞纯化后,胞体呈椭圆形或三角形,有细长的突起,Nuen染色胞核呈棕黄色阳性反应,β3-Tubulin染色细胞胞浆与轴突均呈棕黄色阳性反应。提示实验成功培养出小鼠螺旋神经节细胞。

英文摘要：

BACKGROUND：At present,there are differences in reporting culture condition of cochleal spiral ganglion cells（SGCs） in circle of primary cell culture.Individual method has poor repeatability,and is not beneficial for practical application.OBJECTIVE：To primary culture and evaluate SGCs of neonatal C57 mice.METHODS：Tissues from cochlear modiolus were acquired from neonatal C57 mice by microanatomy.SGCs from cochlear modiolus were cultured by trypsin digestion,differential velocity adherent technique combined with chemicals.Cell growth was observed by inverted phase contrast microscope and hematoxylin and eosin staining.Immunocytochemistry was employed to classify cell types.RESULTS AND CONCLUSION：After purification,the cell body of SGCs from cochlear modiolus was ellipse or triangle,with slender processes in cytoplasm.Nuclei were positive for Nuen（stained brown）.Cytoplasm and axon were positive for β3-Tubulin（stained brown）.These indicated that mouse SGCs were successfully cultured.