中文摘要：

目的研究吡啶霉素（pyridomyein）在吡啶霉素链霉菌（Streptomycespyridomyceticus NRRLB·2517）中生物合成的可能中间体。方法大批量发酵吡啶霉素链霉菌，发酵液经乙酸乙酯萃取、反相硅胶柱层析、凝胶柱层析和半制备高效液相色谱分离获得单体，通过质谱和核磁共振进行波谱分析。结果分离得到目的化合物WT-1。结论经波谱分析，WT-1为一个新结构化合物7-O-α-L-鼠李糖染料木黄酮苷，从WT-1的化学结构及其可能的生物合成机制推测，WT-1与吡啶霉素的生物合成并无直接关联。

英文摘要：

Objective To purify and identify the putative intermediate of the biosynthesis of pyridomycin which is an antimycobacterial cyclodepsipeptide antibiotic produced by Streptomyces pyridomyceticus NRRL B-2517. Method Compound WT-1 was prepared and purified by large scale-fermentation, solvent extraction, RP-18 and LH- 20 column chromatography, and semi-preparative HPLC. Its structure was elucidated by spectroscopic techniques including MS and NMR. Results and Conclusion The target compound WT-1 was purified and identified as a new isoflavone glycoside named 7-O-α-L-rhamnopyranosyl genistein. Structural comparison of WT-1 with the pyridomycin and its putative Type III PKS biosynthetic pathway indicated that the compound WT-1 should not be relevant to the biosynthesis of pyridomycin.