中文摘要：

目的：检测不同Kellgren和Lawrence X线分级骨性关节炎病人膝关节液中微小RNA-140（microRNA-140,miR-140）的表达水平,为进一步研究miR-140在骨性关节炎发生、发展中的作用提供理论依据。方法：收集骨性关节炎病人膝关节液40例[关节炎组,又依据Kellgren和LawrenceX线诊断标准分为Ⅰ级亚组（10例）、Ⅱ级亚组（10例）、Ⅲ级亚组（10例）、Ⅳ级亚组（10例）],非骨性关节炎膝关节液20例（对照组,包括半月板损伤、交叉韧带损伤、外伤）。应用实时荧光定量PCR检测骨性关节炎组及对照组膝关节液中miR-140的含量。以U6snRNA为内参,求出每个样本的Ct值。结果：miR-140在所有关节液标本中都能检测到。与对照组比较,miR-140在关节炎组中的含量下降（P〈0.05）。Ⅳ级亚组miR-140的表达水平与Ⅰ级亚组、Ⅱ级亚组、Ⅲ级亚组比较,差异有统计学意义（P〈0.05）;骨性关节炎的严重程度与miR-140的表达水平呈负相关（r=–0.917,P〈0.05）。结论：人膝关节液中存在miR-140,并且可以方便地应用实时荧光定量PCR进行检测。miR-140在关节炎组膝关节液中的表达水平下调,且与骨性关节炎的严重程度呈负相关,这可能与骨性关节炎的发生、发展有关,并可为潜在的诊断提供预警的分子靶标。

英文摘要：

Objective： To examine the expression of microRNA-140（miR-140） in osteoarthritis（OA） graded by X-ray diagnosis criteria of Kellgren and Lawrence and to provide a theoretical basis for further studing the role of miR-140 in OA development.Methods： Knee synovial fluid specimens were collected in 40 OA patients（the OA group）,the OA group was divided into 4 subgroups according to X-ray diagnosis criteria of Kellgren and Lawrence：gradeⅠsubgroup（n=10）,gradeⅡ subgroup（n=10）,gradeⅢ subgroup（n=10）,and grade Ⅳ subgroup（n=10）.Twenty patients served as a control group（the control group）,including meniscus injury,cruciate ligament injury,trauma patients.The expression level of miR-140 in the knee synovial fluid was detected by real-time fluorescent quantitative polymerase chain reaction（stem-loop RT-qPCR,TaqMan）.U6 snRNA was used as the reference control,and Ct value was calculated.Results： MiR-140 was detected in all knee synovial fluid specimens.The expression level of miR-140 in the OA group was much lower than that in the control groupb（P0.05）.There was significant difference in the expression level of miR-140 between the grade Ⅳ subgroup and the gradeⅠ,gradeⅡ and gradeⅢ subgroups（all P0.05）.The expression levels of miR-140 was negatively related to the severity of OA（r=–0.917,P0.05）.Conclusion： MiR-140 in human knee synovial fluid can be detected conveniently by real-time PCR.The expression level of miR-140 decreases in the knee synovial fluid in OA patients,and is negatively related to the severity of OA.This may be related to the occurrence and development of OA,and can provide early potential diagnostic molecular target.