中文摘要：

目的：观察川续断皂苷Ⅵ（Asperosaponin Ⅵ，ASAⅥ）对脂肪细胞分化的影响及Wnt通路的调节。方法以小鼠骨髓基质细胞系ST-2为研究对象，将其分为对照组、成脂分化组以及4个不同剂量的ASAⅥ组。其中对照组加入溶媒，成脂分化组加入成脂诱导分化试剂，ASAⅥ组除加入成脂诱导分化试剂外，使用不同浓度（10-7、10-6、10-5、10-4 mol/L）的ASAⅥ干预细胞。各组细胞处理5 d后，行油红O染色，观察脂滴形成情况并计算成脂率进行定量分析；荧光定量PCR（FQ-PCR）检测成脂相关基因PPARγ、FABP4和Wnt/β-连环素（β-catenin）通路蛋白β-catenin的mRNA表达水平；Wnt通路抑制剂DKK1干预诱导成脂分化的ST-2细胞5 d，FQ-PCR检测ASAⅥ所调节的PPARγ、FABP4和β-catenin mRNA表达水平。结果与成脂分化组相比，10-5 mol/L和10-4 mol/L ASAⅥ组中分化的脂肪细胞显著减少，10-5、10-4 mol/L ASAⅥ明显抑制PPARγ、FABP4的mRNA表达，但上调β-catenin mRNA表达。DKK1能够逆转ASAⅥ对ST-2细胞成脂分化的抑制作用，促进PPARγ、FABP4的mRNA表达，抑制β-catenin的mRNA表达。结论 ASAⅥ能显著抑制ST-2细胞的成脂分化，这一作用可能是通过Wnt/β-catenin通路的激活所介导的。

英文摘要：

Objective The effect of Asperosaponin Ⅵ（ASAⅥ）on adipocyte differentiation and the involvement of Wnt signal pathway was investigated. Methods The murine bone marrow stromal cell line ST-2 were divided into 6 groups：control group, adipocyte differentiation group, and 4 different doses of ASAⅥgroups. Control group was exposed to the vehicle, adipocyte differentiation group was exposed to adipogenic reagent, and those 4 ASAⅥgroups were treated with different concentration（10-7, 10-6, 10-5, 10-4 mol/L）of ASAⅥafter adipocyte differentiation induction. 5 days later, oil red O staining was performed to calculate adipocyte rate. Then mRNA transcription levels of PPARγ, FABP4 genes andβ-catenin that were Wnt/β-catenin signaling pathway proteins were examined by FQ-PCR. Then Wnt pathway inhibitor DKK1 was supplemented into ST-2 cells treated with 10-4 mol/L ASAⅥfor 5 days. After that FQ-PCR was used to detect whether tran？scription levels of PPARγ, FABP4 andβ-catenin in ST-2 cells were changed. Results Compared with adipocyte differenti？ation group 10-5 mol/L and 10-4 mol/L ASAⅥtreatments greatly down-regulated the number of lipid droplets and markedly inhibited transcription levels of adipocyte characterization transcription factors included PPARγ, FABP4 while up-regulat？ed transcription level ofβ-catenin in ST-2 cells. DKK1 can reverse the inhibitory effect of ASAⅥon adipocyte differentia？tion in ST-2 adipocyte. The transcription levels of PPARγand FABP4 were up-regulated significantly while transcription level ofβ-catenin was inhibited. Conclusion ASAⅥblocks adipocyte differentiation in ST-2 cells which might be medi？ated through activating Wnt/β-catenin signaling pathway.