中文摘要：

目的：探讨制何首乌单味药含药血清对成骨细胞（Ob）功能的影响,并探讨其作用机理。方法：通过血清药理学制备制何首乌单味药含药血清,采用SD新生大鼠头盖骨分离诱导成成骨细胞,应用CCK-8法、碱性磷酸酶（ALP）法,检测制何首乌含药血清对Ob增殖和分化的功能,同时通过RT-PCR方法检测含药血清对Ob成骨相关基因表达的影响。结果：制何首乌含药血清能促进Ob增殖,浓度10%、20%、30%促增殖作用显著（P〈0.01）,组间无浓度相关性;5%和10%的含药血清能下调翻译后水平ALP,但高浓度无此作用。同时,制何首乌含药血清上调成骨相关基因（OC、ALP、Cbfα1）mRNA表达（P〈0.05）。结论：制何首乌单味药含药血清具有刺激体外培养Ob的增殖作用,其作用机理可能与上调Ob成骨相关基因的表达有关。

英文摘要：

Objective ：To study the effect of drug serum of prepared Radix Polygoni Mutiflori on rat osteoblast（Oh） and its mecha- nism. Methods ：The animal serum was prepared by serum pharmacology means. The cells were getting by separating and inducing the SD neonatal rat＇t skull hone. The proliferation and differentiation of Oh were detected by CCK-8 ,alkaline phosphatase （ALP） activity analysis. And RT-PCR method was used to determine the osteogenesis-related genes expression. Results：Compared with control group, the groups with drug serum of prepared Radix Poiygoni Mutiflori, 10% ,20% and 30% had an effect on promotion the proliferation significantly on Ob （P 〈0. 01 ）. There was no concentration-related manner among groups. The 5% and 10% drug serum decreased ALP activity at the post-translation phase compared with control group, but higher doses （20% and 30% ） did not have the same effect. However, drug serum of PR/MIN increased significantly osteogenesis-related genes （ OC, ALP, Cbfcd ） mRNA expression （P 〈 0.05 ）. Conclusion ： The drug serum of prepared Radix Polygoni Muhiflori can stimulate osteoblast proliferation in vitro, and its mechanism may be associated with increasing osteogenesis-related genes expression.