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中文摘要：
目的探讨高迁移率族蛋白B1（HMGB1）对巨噬细胞免疫功能影响的可能受体机制。方法分离培养小鼠腹腔巨噬细胞，HMGB1刺激后，采用激光扫描共聚焦显微镜和流式细胞术检测细胞表面晚期糖基化终末产物受体（RAGE）的表达。实验分为正常对照组、HMGB1组、HMGB1＋抗RAGE抗体组、HMGB1＋rmRAGE／Fc组，分别观察巨噬细胞摄取中性红能力、对L1210细胞的杀伤作用（MTT法）、趋化活性（采用Transwell小室趋化装置）、细胞表面I-A^k抗原表达（流式细胞术）的变化。结果100μg／L的HMGB1作用于巨噬细胞48h后，巨噬细胞表面受体RAGE表达明显上调（P〈0．01）；HMGB1组巨噬细胞吞噬功能、杀伤活性、趋化活性及I-A^k抗原表达均显著高于对照组、HMGB1＋抗RAGE组与HMGB1＋rmRAGE／Fc组（P〈0．01）。结论HMGB1可诱导RAGE表达增强，RAGE是HMGB1作用于巨噬细胞免疫功能的主要受体之一。
英文摘要：
Objective To investigate the potential receptor for high mobility group box-1 protein （HMGB1） on immune function of peritoneal macrophages in mice. Methods The peritoneal macrophages were obtained from female Balb/C mice. After stimulation with 100 μg/L HMGB1 for 48 h, the receptor for advanced glycation end products （RAGE） expression in macrophages was detected by flow cytometry and laser scanning confocal microscopy, respectively. After treated with recombinant mouse RAGE/Fc chimera （rmRAGE/Fc）/HMGB1, anti-RAGE antibody/HMGB1, HMGB1 or nonimmune rabbit igG, the phagocytosis was determined by the neutral red dye uptake, the cytotoxicity to L1210 cells was measured with MTT assay, the chemotaxis was determined by use of Costar Transwell cell culture chamber inserts, and the expression of I-A^k MHC class Ⅱ antigen on macrophages was detected by flow cytometry. Results After stimulation with 100μg/L HMGB1 for 48 h, the RAGE expression in macrophages was markedly increased （P 〈 0.01 ）. The phagocytic activities, cytotoxicity, chemotaxis and Ia antigen expression of macrophages in HMGB1 group were significantly higher than those in other groups （ P 〈 0. 01 ）. Conclusion HMGB1 stimulation can result in up-regulation of RAGE expression on surface of peritoneal macrophages. HMGB1 might modulate immune function of macrophages via RAGE.
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