中文摘要：

为了探索氧化应激活性中间产物丙二醛对小鼠骨髓间充质干细胞成骨分化的影响及机制,体外培养的间充质干细胞经丙二醛处理后,在第4、7、14、21 d分别提取等份细胞进行碱性磷酸酶活性检测,第7 d时提取RNA通过实时定量RT-PCR测定ALP和Runx2/Cbfal mRNA表达,并在第21 d进行von Kossa染色,茜素红染色.研究发现：丙二醛通过降低碱性磷酸酶活性及ALP和Runx2/Cbfal mRNA的表达,抑制矿化骨节形成.这些结果表明：丙二醛可通过抑制ALP和Runx2/Cbfal通路,抑制小鼠骨髓间充质干细胞的成骨分化.

英文摘要：

To explore the role and mechanism of malondialcehyde,a reactive intermediate of oxidative stress,in the osteogenic differentiation of murine bone marrow-derived mesenchymal stem cells（MSCs）,the in vitro cultured MSCs were incubated with malondialdehyde.Then,on the 4th,7th,14th and 21st day,the changes of alkaline phosphatase（ALP） activity were determined respectively.On the 7th day,mRNA was ex-tracted from the cells,ALP and Runx2/Cbfal mRNA expression were analyzed by Q-RT-PCR;and on the 21st day,the osteoblastic phenotype was verified by the von Kossa staining and the Alizarin Red S staining.The results showed that malondialdehyde could reduce the activity of alkaline phosphatase and the mRNA ex-pression of Runx2 and ALP,so as to inhibit the formation of mineralized nodules.The results indicated that the inhibiting mechanism of the osteogenic differentiation of murine MSCs by malondialdehyde may,at least in part,through suppressing key transcription factors of Runx2/Cbfal and ALP.