中文摘要：

steroidal phosphoramidate 3-azido-2,3-dideoxythymidine (AZT ) 和氨基酸酉旨结合的小说被积极、否定的离子 electrospray 电离团 spectrometry 综合并且决定。phosphoramidate 结合的 steroidal 的 MS 破碎行为与 ESI-MS/MS 的双人脚踏车团 spectrometry 一起被调查了。在积极离子 ESI 集体系列有三个典型碎片离子，它是 Na 使内收有 steroidal 一半的损失的离子，从假分子的离子(M+Na ) 的氨基酸酉旨一半 + ，和 phosphoamino 酸甲基酉旨 Na 使内收由 phosphoramidate 的劈开的离子分别地。在否定离子 ESI 集体系列的主要碎片离子是离子(M ? HN3 )? ，离子(M ? AZT ? H )? ，并且离子(M ? steroidal 一半 ? H ) 吗？除假分子的离子以外(M ? H )? 。破碎模式没取决于依附的氨基酸酉旨一半。

英文摘要：

Novel steroidal phosphoramidate conjugates of 3＇-azido-2＇,3＇-dideoxythymidine （AZT） and amino acid esters were synthesized and determined by positive and negative ion electrospray ionization mass spectrometry. The MS fragmentation behaviors of the steroidal phosphoramidate conjugates have been investigated in conjunction with tandem mass spectrometry of ESI-MS/MS. There were three characteristic fragment ions in the positive ion ESI mass spectra, which were the Na adduct ions with loss of steroidal moiety, amino acid ester moiety from pseudo molecular ion （M＋Na）^＋, and the phosphoamino acid methyl ester Na adduct ion by α-cleavage of the phosphora- midate respectively. The main fragment ions in negative ion ESI mass spectra were the ion （M-HN3）^-, the ion （M - AZT- H）^-, and the ion （M-steroidal moiety-H）^- besides the pseudo molecular ion （M-H）^-. The fragmentation patterns did not depend on the attached amino acid ester moiety.