中文摘要：

目的探索组蛋白去乙酰化酶3（HDAC3）基因对胶质瘤细胞U87-MG增殖、凋亡、细胞周期以及迁移的作用。方法人胶质瘤细胞株U87-MG，应用siRNA干扰技术分别导入HDAC3干扰质粒和空载对照质粒，蛋白印迹实验（Wesiemblot）检测干扰效果，应用CCK．8增殖实验、成球实验、AnnexinV-FITC细胞凋亡试剂盒、碘化丙啶染色方法、Transwell小室实验和划痕实验，分别检测HDAC3基因干扰后对胶质瘤细胞株增殖、凋亡、细胞周期和迁移等功能的影响。结果Westernblot结果显示，干扰后细胞株U87-MG中的HDAC3表达明显减弱；CCK-8增殖实验和成球实验，与对照组相比较，胶质瘤细胞株中干扰HDAC3后细胞增殖曲线明显下降，U87-MG对照组和U87．MGSi的14d成球总数目分别为23和7（P〈0．05）；细胞凋亡实验，U87-MG对照组和U87-MGSi的早期凋亡率分别为（1．40±0．29）％和（9．26±2．32）％（P=0．004）；周期实验，U87-MG对照组和U87-MGSi的G0／G1期百分率分别为80．34％和88．37％，（t检验，P〈0．05）；Transwell小室实验和划痕实验，胶质瘤细胞株U87-MG中干扰HDAC3后细胞迁移能力明显下降。结论HDAC3作为一种致癌基因，通过调控细胞G0／G1期参与胶质瘤细胞的增殖、凋亡和迁移。

英文摘要：

Objective To explore the relationship between histone deacetylase 3 （HDAC3）and growth, apoptosis, cell cycle and migration of glioma cells U87-MG. Methods Western blot were used to detect the expression of HDAC3 in glioma cells U87-MG after transfected with siRNA and control RNA. CCK-8 cell proliferation assay, sphere formation, apoptosis assay, transwell migration assay and monolayer wound healing assay were used to explore the effect of the cell growth, apoptosis, cell cycle and migration. Results Western blot results showed that after transfected with siRNA, the expression of HDAC3 in glioma cells were significantly lower. The result of cell proliferation assay showed cell growth of siRNA groups declined compared with control groups. 14 days sphere numbers of U87-MG control group and U87-MG Si group was 23 and 7 （ P 〈 0. 05 ）. The early apoptosis rate of U87-MG control group and Si group were （1.40 ±0. 29）% and （9.26±2. 32）% （ P = 0. 004）. G0/G1 of ug7-MG group and Si group were 80. 34% and 88. 37%. Transwell migration assay and monolayer wound healing assay also deelared that the migration became weaker after transfected. Conclusions HDAC3 partieipated in cell growth, apoptosis, migration of glioma cells through G0/Gl arrest as an oneogene.