中文摘要：

【目的】了解抗条锈小麦受条锈菌侵染后的蛋白表达变化情况,采用比较蛋白质组学技术鉴定接种条锈菌小种CY32后的抗条锈小麦品系Taichuang29＊6/Yr5和同时期未接种对照之间的差异蛋白。【方法】提取接种48h和同期未接种小麦的叶片蛋白,进行双向电泳分离和分析,选取差异显著的蛋白点进行MALDI-TOF质谱分析及数据库搜索鉴定。【结果】发现13个体积百分比变化大于1.5倍,符合t检验（P〈0.05）的显著差异蛋白点,通过MALDI-TOFMS获得了这些差异蛋白点的肽指纹图谱,经数据库搜索,共鉴定出11个蛋白,包括Pathogenesis-related homeodomain protein,beta-glucosidase,glutathione transferase等。【结论】经蛋白质功能分析,推测小麦叶片中这些差异蛋白可能与条锈菌小种CY32的侵染有关。

英文摘要：

[Objective] To investigate the biochemical changes of wheat resistance to stripe rust after infected with the stripe rust, a resistive line of wheat （Taichung29＊6/Yr5） infected with stripe rust CY32 was compared to mock-inoculated wheat by comparative proteomics technology [ Method] Proteins were extracted from leaves infected with a virulent race of stripe rust CY32 and control group after 48 hours of inoculation, and then separated by using two-dimensional electrophoresis （2-DE）. The differential protein spots were selected for MALDI-TOF MS analysis and database searching. [Result] By using 2-DE, 2 uniquely expressed spots, 5 up-regulated spots and 6 down-regulated spots were observed in Taichung29＊6/Yr5 leaves inoculated with stripe rust CY32. Total of 11 proteins, including pathogenesis-related homeodomain protein, beta-glucosidase, glutathione transferase etc, were identified. [Conclusion] Through analysis of protein function, it is presumed that these differential proteins are correlated with the infextation of stripe rust race CY32.