中文摘要：

目的探讨生长抑素（SST）基因在子宫内膜样腺癌组织及子宫内膜癌细胞系中的表达水平，以及过表达该基因对子宫内膜癌Ishikawa细胞增殖及侵袭能力的影响。方法选取正常子宫内膜、子宫内膜样腺癌及子宫内膜浆液性癌组织切片，采用免疫组织化学方法检测SST在各组织切片中的表达。收集手术新鲜标本，病理诊断均为子宫内膜样腺癌，按2009年FIGO标准病理分级，其中高分化（G1）7例、中分化（G2）6例、低分化（G3）5例，提取RNA，采用实时荧光定量PCR方法检测SST的表达情况。选取Ishikawa、HEC-1A及KLE细胞系，采用实时荧光定量PCR及Westernblot方法检测SST表达水平。以SST过表达慢病毒pLVX-SST（Ish-SST组）及空载慢病毒pLVX（Ish-ctr组）转染Ishikawa细胞，荧光显微镜下观察转染效率。Westernblot检测两组Ishikawa细胞中SST蛋白表达水平。采用CCK-8及Transwell实验检测两组Ishikawa细胞的增殖及侵袭能力。结果免疫组织化学结果显示，与正常子宫内膜相比，SST在子宫内膜样腺癌及子宫内膜浆液性癌中的表达显著增加。KLE组SST的mRNA及蛋白水平较Ishikawa组及HEC-1A组高（P＜0.05），HEC-1A组SST的mRNA及蛋白水平高于Ishikawa组（P＜0.05）。在子宫内膜样腺癌中，与G1和G2相比，SST的表达在G3中显著增加（P＜0.05）。在Ishikawa细胞传代2代后，Ish-SST组SST蛋白表达水平显著高于Ish-ctr组。SST过表达Ishikawa细胞增殖及侵袭能力与对照组差异无统计学意义（P＞0.05）。结论SST在低分化子宫内膜样腺癌中呈高表达；SST过表达不能增加Ishikawa细胞的增殖及侵袭能力。

英文摘要：

Objective To investigate the expression levels of somatostatin(SST)gene in endometrial adenocarcinoma tissues and cell lines,and the effects of over-expression of SST gene on the proliferation and invasion of endometrial cancer cell line Ishikawa in vitro.Methods Tissue sections of normal endometrium,endometrioid adenocarcinoma and uterine papillary serous carcinoma were selected to detect the expressions of SST by immunohistochemical method.The total RNA was extracted from fresh specimens that were confirmed as endometrioid adenocarcinoma.According to FIGO staging,samples included G1(7cases),G2(6cases)and G3(5cases)of endometrioid adenocarcinoma.The SST expression levels were detected by real-time PCR.Three endometrial cancer cell lines,Ishikawa,HEC-1A and KLE,were selected and the expression levels of SST were detected by real-time PCR and Western blot assay.Transfection was performed with pLVXSST and pLVX.The transfection efficiency was observed by fluorescence confocal microscopy.The protein levels of SST were detected by Western blot assay.The assays of CCK-8and transwell were applied to examine variations in cell proliferation and invasion.Results Immunohistochemical results showed that SST expression was increased in endometrioid adenocarcinoma and uterine papillary serous carcinoma compared with that of normal endometrium.Real-time PCR showed that SST expression was significantly increased in G3compared with that of G1and G2in endometrioid adenocarcinoma(P<0.05).No matter mRNA or protein,SST levels were significantly increased in endometrial cancer cell line KLE compared with those of Ishikawa and HEC-1A,and the expression levels of SST mRNA and protein were significantly increased in HEC-1A group than those of Ishikawa group(P<0.05).The expression of SST protein was significantly higher in the group of Ish-SST after2generations compared with that of Ish-ctr group.There were no significant differences in cell proliferation and invasive ability after over-expression of SST between Ishikawa cell group and c