以分别嫁接在杜梨和豆梨上的砂梨品种‘丰水’为试材,研究了2008和2009年11~12月气温变化和不同砧木对‘丰水’梨叶芽休眠进程的影响,分析叶芽中游离态和束缚态内源多胺种类和含量的变化,结果表明:嫁接在豆梨上的‘丰水’叶芽自然休眠结束的时间要比嫁接在杜梨上的‘丰水’叶芽早10d左右,且游离态和束缚态腐胺(Put)、戊二胺(Cad)、己二胺(Hex)、亚精胺(Spd)和精胺(Spm)5种内源多胺含量开始升高的时间与供试材料叶芽自然休眠结束的时间一致,表明梨叶芽的休眠进程与砧木种类和多胺代谢有密切关系,尤其是与束缚态多胺含量变化的关系更为密切。
'Housui' pear trees(Pyrus serotina cv.'Housui') grafted on P.betulaefolia and P.calleryana separately were used as experimental materials in this article.The effects of temperature variations at orchard from November to December and different rootstocks on the dormant process of leaf buds were compared,and content changes of free and bound polyamines in leaf buds of 'Housui' pear trees were analyzed during the dormant periods in 2009 and 2010.The results showed that the time when the rate of leaf bud germination appeared over 50% in 'Housui' pear trees grafted on P.calleryana was 10 d earlier than that in 'Housui' pear trees grafted on P.betulaefolia.Also the time when the contents of five kinds of endogenous polyamines,free and bound putrescine(Put),cadaverine(Cad),hexanediamine(Hex),spermidine(Spd) and spermine(Spm),began to increase was consistent with the period that dormancy ended in the leaf buds of the experimental materials.The research indicates that the dormant process changes of pear buds are influenced by the rootstock varieties and the polyamine metabolism.It seems that the effects of the content changes of bound polyamines in the pear buds on the dormant process are clearer than those of free polyamines.