中文摘要：

本研究根据茎瘤芥转录组测序筛选获得了一个MYB基因片段。通过RACE的方法,克隆得到了两条长度分别为975bp和864bp的全长序列,分析发现可能为茎瘤芥MYB基因的两种不同选择性拼接形式,并命名为BjMYB1-3和BjMYB1-4。对BjMYB1-3和BjMYB1-4做组织表达分析,发现这两个转录本在茎中特异表达。针对BjMYB1-3和BjMYB1-4的亚细胞定位分析,显示BjMYB1-4定位于细胞核,BjMYB1-3部分定位于细胞核。构建BjMYB1-3和BjMYB1-4的超表达载体转化拟南芥,结果显示相比于野生型拟南芥,BjMYB1-3超表达转基因株系生长状态更好,而BjMYB1-4超表达转基因株系没什么明显变化,说明BjMYB1-3在植株的生长发育过程中起着一定作用。

英文摘要：

A fragment of MYB gene was figured out based on transcription analysis of different periods of stem swelling. Two eDNA sequences, 975bp and 864bp, were cloned by rapid amplification of eDNA ends. Moreover, these two genes, namely BjMYB1-3 and BjMYB1-4, may be the two different alter- native splicing patterns of BjMYB1 gene. By analysis the tissue expression of BjMYB1-3 and BjMYB1-4, they were particularly expressed in stem. An analysis of the subcellular localisation of the proteins indicated that BjMYB1-4 was present only in the nucleus, while the BjMYB1-3 GFP fusion protein was partially localised to the nucleus. By constructing the overexpression vector of BjMYB1-3 and BjMYB1-4 and conducting the genetic transformation of Arabidopsis thaliana, we found that the plants of BjMYB1-3-overexpression showed obviously higher plant height at flowering stage, while the BjMYB1-4-overexpression plants showed no obvious difference. The result showed that these two transcripts played an important role in the growth and development of plant.