中文摘要：

目的：研究黄芩苷/栀子苷配伍（7：3）对脑缺血再灌注损伤大鼠神经功能损伤的修复作用及作用机制。方法：采用改良线栓法制作脑缺血再灌注损伤模型,造模后24 h,采用平衡木试验、大鼠抓力测定法进行评价神经功能缺损,ELISA法观察脑组织中Na^＋、K^＋-ATP酶、Ca^2＋、Mg^2＋-ATP酶活性大小,尼式（Nissl）染色法检测大鼠冠状脑区皮质神经元的形态和数量,免疫组织化学检测脑组织中水通道蛋白-4（（aquaporin-4,AQP-4）、胶质纤维酸性蛋白（glial fibrillary acidic protein,GFAP）的表达。结果：与模型对照组相比,黄芩苷/栀子苷配伍治疗组能降低神经功能损伤,减少进入血管外及细胞间的颗粒、减轻血管内皮细胞水肿,增加Nissl阳性细胞的数量,升高脑组织中Na^＋、K^＋-ATP酶、Ca^2＋、Mg^2＋-ATP酶活力,下调AQP-4蛋白、GFAP蛋白表达,其中,黄芩苷、栀子苷配伍（42＋18 mg/kg.d）组有显著性差异。结论：黄芩苷/栀子苷配伍能够减轻CIRI脑水肿,对大鼠缺血再灌注脑损伤的神经保护作用可能与增加Na^＋、K^＋-ATP酶、Ca^2＋、Mg^2＋-ATP酶活力,下调AQP-4及GFAP蛋白表达有关。

英文摘要：

Objective： This study is to investigate the protection effect and mechanism of the compatibility of baicalin and gardenoside （ 7 ： 3 ） on the recovery of neurol gical function injury after cerebral ischemia reperfusion injury （CIRI） in rats. Method： The model of CIRI made by im- proved Line plug method, after 24 h, made neural function defect scale with grasping force measurement and Longa scores and balance beam test, tested the dynamic size of Na^ ＋ ,K ^＋ -ATP enzymes and Ca^2 ＋ , Mg^2 ＋ -ATP enzymes by ELISA, morphology and number of neurons in cere- bral cortex was observed by Nissl staining method, immunohistochemical staining observed the expression of GFAP and AQP-4. Result： Compared with the model group, baicalin and geniposide group can recover the neurological damage, reduction of lanthanum particles into the blood vessels and the cells of the cells, reduce the vascular endothelial cell edema, increase the activity of Na^＋ , K ^＋ ATPase and Ca^2 ＋ , Mg^2 ＋- ATPase in brain tissue, increase the number of Nissl positive cells in cerebral cortex, down-regulate the expression of GFAP and AQP-4, ba- icalin and geniposide group（42 ＋ 18 mg/kg, d）have significant differences. Conclusion： The baicalin and gaurdenoside （7：3） can recover the neurological damage might to attributed to the increase the activity of Na ^＋ , K ^＋ ATPase and Ca^2＋ , Mg^2＋ -ATPase, down-regulate the ex- pression of GFAP and AQP-4, antagonize energy metabolism disorder.