中文摘要：

目的：高热量物质的过度摄入是导致机体代谢紊乱,诱发2型糖尿病等代谢性疾病的主要原因,本文通过比较高果糖、高脂及高果糖高脂混合喂饲对小鼠体内能量代谢的影响,探索饮食诱发代谢紊乱性疾病的可能发病机制。方法：采用20%高果糖水,60%高脂饲料,及二者混合方式饲养C57BL/6小鼠3个月后,观察各组小鼠24小时内氧气消耗量,二氧化碳生成量,呼吸商及能量消耗的改变。结果：不同饮食喂饲3个月,与对照组小鼠相比,高果糖组、高脂组、及高果糖高脂组小鼠均表现出明显的肝内脂质蓄积,氧气消耗量增加,呼吸商下降,能量消耗增加。结论：过剩的高热量物质摄入导致机体内物质代谢、能量代谢发生改变,糖代谢受损,脂代谢增强,能量代谢方式从糖氧化为主转变为脂氧化供能。

英文摘要：

Objective： Excess energy intake is a main causal reason for metabolic disorders. To investigate the possible pathogenesis mechanism of metabolic dysfunction, the effects of different high energy foods on energy metabolism were compared. Methods： Oxygen consumption, carbon dioxide production, respiratory quotient and energy expenditure within 24 hours were observed in C57BL/6mice fed with 20% fructose water, 60% high fat diet, or the combination diet. Results： After 3 month feeding, all of the three energy-dense groups showed lipid accumulation in liver, respiratory quotient decrease, and energy expenditure increase compared with control group. Conclusions： Excess energy intake led to glucose metabolism injury and lipid metabolism increase. The pattern of energy metabolism was changed from glucose oxidation into lipid oxidation as energy supplier.