中文摘要：

采用SSR、EST-SSR和SRAP 3种分子标记鉴定8种软枣猕猴桃区试品种种子纯度,结果表明,EST-SSR引物多态性、稳定性好,可用于后续试验;8种软枣猕猴桃区试品种中DNA位点纯合率最高的是‘LD241’和‘LD126’,在受检的53个位点上,未检测到非纯合位点,DNA位点纯合率100%,‘宝贝星’最低（92.5%）;而纯度最高的是‘LD126’和‘宝贝星’（95.8%）,纯度最差的是‘绿珍珠’（70.8%）,说明种子的纯度与DNA位点纯合率的相关性并不大,进一步明确种子生产纯度（去除异型株的纯度）与分子遗传纯度2个概念,为育种及减少农艺性状差异（一致性、稳定性）提供重要依据。

英文摘要：

Using three methods of molecular markers sucha as SSR,EST-SSR and SRAP to identify the seed purity of eight Actinidia arguta;the results showed that polymorphism and stability of ESTSSR primer was better,which could be used for the subsequent experiments;LD241,LD126 had the highest homozygous DNA locus among the eight regional trial varieties（100%）,because no homozygous DNA loci was detected at the 53 sites,the lowest one was Baby star（92.5%）;the LD126 and Baobei-star had the highest seed purity（95.8%）,Green Pearl（70.8%）was a little lower,it showed that there was no correlation between the seed purity and homozygous DNA locus,the two concepts of purity of seed production（removal of heterotype individuals）and the homozygous DNA locus（genetic purity）were further clarified.So the results could provide the important basis for seed breeding and reducing differences of agronomic characters and so on.