中文摘要：

目的探讨姜黄素对人脑胶质瘤U251细胞侵袭与迁移的抑制作用。方法采用四甲基偶氮唑蓝法筛选姜黄素实验浓度;采用细胞划痕实验观察姜黄素作用0、6、12和24 h后胶质瘤U251细胞的迁移情况,并且测量24 h后迁移的距离;采用Transwell实验观察穿膜细胞数,以判断细胞的侵袭能力;采用Western blotting实验检测胶质瘤U251细胞中侵袭相关蛋白基质金属蛋白酶2（matrix metalloproteinase-2,MMP2）和基质金属蛋白酶9的表达。结果四甲基偶氮唑蓝法筛选的姜黄素浓度为10和20μmol·L-1;与空白对照组相比,在姜黄素作用24 h后,胶质瘤U251细胞迁移的距离和穿膜细胞数皆明显减少,且与药物浓度呈正相关（P〈0.01）;与空白对照组相比,姜黄素组胶质瘤U251细胞内基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达明显下降（P〈0.01）,在姜黄素10和20μmol·L-1组基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达差异明显（P〈0.05）。结论姜黄素能够抑制人脑胶质瘤U251细胞的迁移与侵袭,可能是通过下调细胞内基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达引起的。

英文摘要：

OBJECTIVE To investigate the inhibitory effect of curcumin on invasion and migration of human glioma U251 cells.METHODS Curcumin experimental concentration was screened by MTT.Through cell scratch assay,migration distance of glioma U251 cells was observed after using curcumin for 0,6,12 and 24 h,migration distance of cells was measured after treatment with curcumin for 24 h.Through transwell assay,transmembrane cell number was recorded and to judge invasive ability of cells.Through Western blotting assay,invasion related proteins matrix metalloproteinase 2（ MMP2） and MMP9 were detected in the glioma U251 cells.RESULTS Screening curcumin concentration was 10 and 20 μmol·L-1by MTT.Compared with the control group,the migration distance and transmembrane cell number of glioma U251 cells decreased significantly after culturing cells for 24 h with curcumin,which were positively correlated with the concentration of drug（ P 0.01）.Compared with the control group,the expression of MMP2 and MMP9 proteins of glioma U251 cells decreased significantly in curcumin groups（ P 0.01）.Compared with the curcumin10 μmol·L-1group,the expression of MMP2 and MMP9 proteins decreased significantly in the curcumin 20 μmol · L-1group（ P 0.05）.CONCLUSION Curcumin can inhibit the migration and invasion of human glioma U251 cells,and it is possible that the result is relevant to the down-regulation of the expression of MMP2 and MMP9 proteins.