中文摘要：

背景血脂异常是年龄相关性黄斑变性（AMD）的发病因素之一，AMD的治疗方法一直是研究的热点。目的观察健脾祛瘀中药制剂对血脂异常ApoE基因缺失（ApoE-／-）小鼠视网膜和Bruch膜的治疗作用。方法采用随机数字表法根据小鼠体质量分层随机分组。将36只2月龄ApoE-／-小鼠分为普通饮食组、高脂模型组及健脾祛瘀治疗组，每组12只。高脂模型组及健脾祛瘀治疗组小鼠均用高脂饲料喂养4个月，健脾祛瘀治疗组小鼠于6月龄开始给予健脾祛瘀制剂灌胃，每次0．3ml，每Et2次，持续1个月；普通饮食组和高脂模型组小鼠同时间点开始用等量生理盐水灌胃，从6月龄开始3个组均给予普通饲料喂养。于小鼠7月龄时抽取股静脉血检测各组小鼠血浆总胆固醇（Tc）、低密度脂蛋白（LDL）和血浆甘油三脂（TG）浓度，抽血后以过量麻醉法处死，制备视网膜组织切片并行Masson染色，光学显微镜和透射电子显微镜下观察视网膜和Bruch膜的组织形态学改变。采用Mias2000型图像分析系统半定量检测各组小鼠视网膜外核层细胞、视网膜色素上皮（RPE）层细胞数及Bruch膜厚度。结果7月龄健脾祛瘀治疗组小鼠TC、LDL和TG浓度分别为（6．47±0．49）、（1．46±0．10）、（0．62±0．21）mmol／L，比同龄高脂模型组小鼠的（10．53±0．30）、（1．90±0．13）mmol／L和（1．15±0．29）mmol／L以及普通饮食组小鼠的（9．63±0．18）mmol／L、（1．12±0．15）、（0．88±0．21）mmol／L均明显降低，差异均有统计学意义（P〈0．05～0．01）。7月龄高脂模型组小鼠及同龄普通饮食组小鼠外核层和RPE层细胞排列紊乱，细胞萎缩明显，Bruch膜纤维分叉，高脂模型组部分小鼠可见玻璃膜疣；而同龄健脾祛瘀治疗组小鼠外核层和RPE层细胞排列较整齐，细胞萎缩减轻，Bruch膜纤维未见分叉，未见玻璃膜疣。7月龄健?

英文摘要：

Background Dyslipidemia is one of the major causes of age-related macular degeneration （AMD）. At present,the study of the preventive and treating methods of AMD is still a hot spot. Objective The purpose of this study was to observe the effect of tonifying the spleen and promoting blood circulation on the retina and Brueh membrane in apolipoprotein E-deficient （ApoE-/-） mice with dyslipidemia. Methods Thirty-six ApoE-/- mice aged 2 months were randomly divided into the normal diet group,high fat diet group and medicine group. A diet with a higher content of fat was given for 5 consecutive months to the mice of the high fat diet group and medicine group,and in the last month, a concoction that tonifies the spleen and promotes blood circulation was gavagely administered in the medicine group,and an equivalent volumes of normal saline solution was administered in the same way in the normal diet group and high fat diet group. Total plasma cholesterol （TC） ,low density lipoprotein （LDL） and triglyceride （TG） were detected by （ELISA？ Name of assay？） using the 7170 Hitachi automatic biochemical analyzer,and morphological changes of the retina and Bruch membrane were examined by light microscopy and transmission electron microscopy. The number of outer nuclear layer （ONL） ceils, retinal pigment epithelial （RPE） cells and the thickness of the Bruch membrane were examined by semi-quantitative histopathology with the Mias 2000 Imaging Analyzer System. Results The concentrations of TC, LDL and TG were （6.47 ±0.49） mmol/L, （ 1.46± 0. 10）mmol/L and （0.62 ±0.21 ）mmol/L, respectively, in 7-month-old mice of the medicine group, showing a significant reduction in comparison with （10.53±0.30） mmol/L, （ 1.90±0.13 ） mmol/L, （ 1. 15±0.29） mmol/L of the high fat diet group, and （ 9.63± 0. 18 ） mmol/L, （ 1.12±0. 15 ） retool/L, （ 0.88 ± 0.21 ） mmol/L in the normal diet group （P〈0.05-0.01）. The disorder and atrophy of ONL and RPE cells, divergence of fi