中文摘要：

微生物降解是多环芳烃从土壤和水环境中消失的主要途径。本文从加油站废油排放口附近被污染土壤中筛选到两种降解萘的混合菌H1、H2并从中分离出一株纯菌C1。研究发现，利用纯无机盐培养基加萘作为唯一碳源要比加入其它营养物质的培养基对于筛选萘降解菌更为有利。H1、H2和C1均能以萘为唯一碳源生长，在萘初始浓度为1000mg/L的纯无机盐培养基中培养40h后，培养液中微生物数量分别增长10倍以上。利用气相色谱测得萘的降解率都达到99．6％以上。

英文摘要：

Microbial degradation has been suggested as the best way to remove polycyclic aromatic hydrocarbons （PAHs） from contaminated environment, in this study, two naphthalene-degrading mixed cultures H1 and H2 were enriched from the petroleum-contaminated soil around a gas station, and one preponderant pure bacterial strain C1 utilizing naphthalene as a sole carbon and energy source was isolated from the mixed cultures. The results indicate that culture medium with naphthalene as a sole carbon and energy source is better than with other carbon nutriment in screening microorganism capable to degrade naphthalene. It was found that after 40h incubation in mineral salt medium with an initial naphthalene concentration of 1000 mg·L^-1, the cell numbers of H1, H2 and C1 had increased more than ten times and the degrading rates of naphthalene were over 99.6%.