中文摘要：

电离放射(红外) 直接并且间接地引起严重细胞的损坏并且破坏 RNA 完整。RNA 海滨裂缝是红外引起的损坏的最经常的类型。RNA 损坏涉及退化疾病的发展，包括 Alzheimers 疾病和 Parkinsons 疾病。然而， mRNA 损坏的机制和任何产生 pathophysiological 结果糟糕被理解。这是因为有敏感工具的缺乏监视，部分随机损坏发生在 RNA，在给定的 RNA 的特别 RNA 海滨裂缝损坏。在这个工作，用反向的抄写聚合酶链反应(RT-PCR ) 在以后的一个方法 poly RNA 的 3 结束的(A) 增加在特定的 RNA 决定 RNA 海滨裂缝损坏由 poly，(A) 聚合酶被开发了。在从对待红外的 HeLa 房间的特定的 mRNAs，包括的 ABL1， TP53， GADD45A 和 ATR 的损坏的层次被检验。海滨裂缝在检验的所有 mRNAs 被检测。学习 poly 基于 3 结束提供一个新奇、敏感的方法监视 RNA 的(A) 跟踪 RT-PCR 海滨裂缝损坏。

英文摘要：

Ionizing radiation （IR） causes severe cellular damage both directly and indirectly and disrupts RNA integrity. RNA strand breaks are the most frequent type of damage caused by IR. RNA damage is involved in the development of degenerative diseases, in- cluding Alzheimer＇s disease and Parkinson＇s disease. However, the mechanism of mRNA damage and any resulting pathophysi- ological outcomes are poorly understood. This is partly because there is a lack of sensitive tools to monitor damage randomly occurring in RNA, especially RNA strand break damage in a given RNA. In this work, a method using the reverse transcription polymerase chain reaction （RT-PCR） after poly（A） addition to 3＇-end of RNA to determine RNA strand break damage in a specific RNA by poly（A） polymerase has been developed. The levels of damage in specific mRNAs, including ABL1, TP53, GADD45A and ATR from IR-treated HeLa cells were examined. Strand breaks were detected in all mRNAs examined. The study provides a novel and sensitive method based on 3＋-end poly（A）-tailing RT-PCR to monitor RNA strand break damage.