中文摘要：

以产复合酶益生菌黑曲霉ANO1为出发菌株。经多次N^＋注入诱变处理．结合平板透明圈法定向选育，得到高产变异菌株ANO2。结果显示，出发菌株ANO1酸性蛋白酶、纤维素酶和果胶酶的酶活显著提高，分别由71．6IU／g、141．7IU／g和264．8IU／g提高到996．5IU／g、940．4IU／g和906．5IU／g。变异菌株ANO2经传5代培养，产酶特性稳定。同时对原菌株和变异菌株的生物学特性进行了相关的研究，结果表明变异菌株的生物量和产酶量成正相关。

英文摘要：

A mutated strain ANO2 with high yield of compound enzymes was obtained from the strain ANO1 of Aspergillus niger by N^＋ implantation, and screened by using transparent zones of agar plate screening techniques. The results showed that activities of acid-protease, Cellulase and Pectinase in A spergillus niger were raised signifcantly from 71.6 IU/g, 141.7 IU/g and 264.8 IU/g to 996.5 IU/g, 940.4 IU/ g and 906.5 IU/g respectively. Characteristics of enzymes production in mutated strain Aspergillus niger ANO2 were stabile by 5 times subculture.Biological characteristics of the original strain ANO1 and the mutant strain ANO2 were researched. Biomass and enzyme activity of the mutant strain ANO2 were increasing relativity.