中文摘要：

热休克反应是机体中一个重要的内源性保护机制，但其对TNF电诱导的单核／巨噬细胞迁移有无影响目前尚不清楚．采用酶联免疫吸附实验观察TNF-α（20μg/L）刺激RAW264．7巨噬细胞4h后炎症因子IL-1β、IL-6、IL-15的表达情况；Western blot验证热休克预处理诱导热休克蛋白表达的增加；利用细胞趋化实验观察热休克预处理（42℃，1h）对TNF哪所致巨噬细胞迁移的影响．研究发现，TNF-α可明显促进RAW264．7细胞株中IL-1β、IL-6、IL-15等炎症因子的释放；热休克预处理诱导热休克蛋白HSP70、HSP90、HSP25表达增加；细胞趋化实验发现TNF-α处理的RAW264．7细胞迁移能力较正常对照组明显增强，而热休克预处理组巨噬细胞的迁移能力较单纯TNF—α处理组明显减弱．上述结果表明，热休克预处理抑制TNF-α所致巨噬细胞的迁移．

英文摘要：

In order to elucidate the effect of heat shock response on TNF-α induced RAW264.7 macropha- ges migration, enzyme linked immuno-sorbent assay（ELISA） was used to observe if TNF-α（20 μg/L） could induce the expression of inflammatory factors IL-1β、IL-6、IL-15; Western Blot assay was used to confirm that Heat shock pretreatment（42 ℃, 1 h） could induce the expression of HSPs. Chemotaxis assay was used to observe the effect of TNF-a and heat shock response on RAW264.7 macrophages migration. It was found that TNF-α can induce the expression of inflammatory factors such as IL-1β、IL-6、IL-15; Heat shock response can induce the expression of HSP70, HSP90, HSP25; The migration of macrophages increased when treated with TNF-α, but heat shock response inhibited such effect. The result showed that heat shock response could inhibit RAW264.7 maerophages migration induced by TNF-α.