中文摘要：

目的：研究银杏叶提取物（EGB）对I型糖尿病心肌病大鼠心肌TGF-β1和collagen表达及间质纤维化的影响.方法：（1）在体实验,取30只雄性SD大鼠随机分为正常对照组（CON组）、糖尿病组（DM组）和EGB干预组（EGB组）;用链脲佐菌素诱导Ⅰ型糖尿病大鼠模型,于第12周末测定大鼠体重、左心室重量、血糖、糖化血红蛋白和血胰岛素;用超声心动图仪检测左室舒张末期容积（LVEDV）、左室收缩末期容积（LVESV）、左室射血分数（LVEF）和每搏射血量（SV）;用天狼星红染色、免疫组织化学染色及RT-PCR方法分别检测左心室心肌胶原含量、转化生长因子β1（TGF-β1）蛋白表达及TGF-β1、procollagen Ⅰ和collagen Ⅲ mRNA表达.（2）离体实验,分离培养SD大鼠乳鼠心肌细胞并分别用低糖（LG组）、高糖培养基（HG组）和EGB干预（HG＋EGB组）;RT-PCR方法检测各组心肌细胞TGF-β1、procollagen Ⅰ和collagen Ⅲ 的mRNA表达.结果：（1）与CON组相比,DM组大鼠血糖（P＜0.01）、糖化血红蛋白（P＜0.05）、左心室重量指数（P＜0.01）、心肌间质胶原纤维含量（P＜0.05）以及心肌组织内TGF-β1（P＜0.05）、procollagen Ⅰ（P＜0.05）和collagen III（P＜0.05）表达均明显升高,而血胰岛素水平（P＜0.01）、LVEDV （P＜0.01）和SV （P＜0.01）明显降低;EGB干预治疗后,与DM组相比,EGB组糖尿病大鼠血糖（P＜0.01）、糖化血红蛋白（P＜0.05）、左心室重量指数（P＜0.05）、心肌间质胶原纤维含量（P＜0.05）以及心肌组织内TGF-β1（P＜0.05）、procollagen Ⅰ（P＜0.05）和collagen Ⅲ（P＜0.05）表达均明显降低,血胰岛素水平（P＜0.05）、LVEDV （P＜0.05）和SV （P＜0.05）明显升高.（2）与LG组比较,HG组心肌细胞TGF-β1（P＜0.01）、procollagen Ⅰ（P＜0.01）和collagen Ⅲ（P＜0.01） mRNA表达明显升高;而EGB干预后,与HG组比较,EGB组心肌细胞TGF-β1（P＜0.01）?

英文摘要：

AIM： To study the effects of Ginkgo. biloba extract （EGB） on myocardial TGF-β1 and collagen expression and interstitial fibrosis in type Ⅰdiabetic cardiomyopathy rats. METHODS： Thirty male SD rats were randomly divided into normal control group （CON）, diabetes mellitus group （DM） and EGB treatment group （EGB）. Streptozocin was intraperitoneally injected into the animals in the latter 2 groups to induce typeⅠ diabetic rat model. The rats in EGB group were intraperitoneally injected with EGB. At the end of the 12th week, the body weight of each rat and its left ventri- cular weight, blood glucose, glycosylated hemoglobin and serum insulin concentration were measured. The left ventricularend-diastolic volume （LVEDV）, the left ventricular end-systolic volume （LVESV）, the left ventricular ejection fraction （LVEF） and the stroke volume （SV） were determined by echocardiography. The content of collagen in left ventricular myocardium, and the expression of transforming growth factor β1 （TGF-I31）, procollagen type I and collagen type Ⅲ were assayed by Sirius red staining, immunohistochemical sthining and RT-PCR, respectively. Left ventricular myocardial cells of the neonatal SD rats were isolated and cultured in vitro with low-glucose culture medium （LG group）, high-glucose culture medium （ HG group） or high-glucose culture medium plus EGB （ HG ＋ EGB group）. The mRNA levels of TGF-t51 , procollagen type Ⅰ and collagen type Ⅲ were detected by RT-PCR. RESULTS： Compared with CON group, blood glucose,glycosylated hemoglobin, left ventricular weight index, the content of collagen, and the expression of TGF-β1, procollagen type Ⅰ and collagen type Ⅲ in left ventricular myocardial tissues of DM group were significantly increased, while the levels of blood insulin, LVEDV and SV were significantly decreased. However, compared with DM group, blood glucose, glyco- sylated hemoglobin, left ventricule weight index, the content of collagen, and the expression level