中文摘要：

目的：观察过度训练对大鼠腹膜巨噬细胞吞噬功能的影响及胰岛素样生长因子1（IGF-1）和机械生长因子（MGF）在其中所起的作用。方法：8周龄健康雄性wstar大鼠16只，随机分为安静对照组（control）和过度训练组（overtraining）。过度训练组进行11周递增负荷跑台训练。最后1次训练后36h，记录体重变化并测定血液中血红蛋白和睾酮含量。断头处死大鼠并分离纯化腹膜巨噬细胞，中性红法测定巨噬细胞吞噬功能，荧光定量PCR技术测定IGF-1和MGF基因表达。离体状态下用不同浓度的IGF-1或MGF与巨噬细胞孵育2h，观察IGF-1和MGF对巨噬细胞吞噬功能的影响。结果：与安静对照组相比，过度训练组大鼠体重、血红蛋白和睾酮含量均显著降低，分别下降了约19．3％（P〈0．01）、13．5％（P〈0．01）、55．3％（P〈0．01）。过度训练组巨噬细胞摄取中性红能力显著低于安静对照组，下降约27％（P〈0．05）；过度训练组巨噬细胞IGF-1 mRNA水平约为安静对照组的21倍（P〈0．01），MGFmRNA水平约为安静对照组的92倍（P〈0．01）；IGF-1对巨噬细胞吞噬功能无显著影响，各浓度组与未加药组相比均无显著性差异（P〉0．05）；MGF呈浓度依赖性的抑制巨噬细胞吞噬功能，与未加药组相比，10ng／ml、50ng／ml组有显著性差异（P〈0．05），100ag／ml、200ng／ml组有极显著性差异（P〈0．01），各浓度组间比较无显著性差异（P〉0．05）。结论：过度训练抑制巨噬细胞吞噬功能；过度训练诱导产生的MGF可能在过度训练抑制巨噬细胞吞噬功能中发挥关键作用。

英文摘要：

Objective To investigate the effects of overtraining on the phagocytosis of peritoneal macrophages and the roles of IGF-1 and MGF in this process. Methods： 16 male wistar rats were randomized to 2 groups：sedentary group （n= 8） and overtraining group （n= 8）. Overtraining group was training in standard treadmill with an increasing load for 11 weeks. 36 h after the last session of exercise, record the body weight, blood was sampled at the retro-orbital sinus to determine the contents of hemoglobin and testosterone. Rats were sacrificed by decapitation, and then peritoneal macrophages were isolated and purified. The phagocytosis and mRNA levels of IGF-1 and MGF were measured by the uptake of neutral red and real-time PCR respectively. In addition, we evaluate the effects of IGF-1 and MGF peptide on phagocytosis of peritoneal macrophages in vitro （MФs were exposed to different concentrations of IGF-1 or MGF peptide for 2 h）. Results .. There was a decline of body weight （ by 19.3% ）, hemoglobin （by 13.5 % ） and testosterone （by 55.3 % ） in overtraining group when compared with control group （P 〈 0. 01 ）. Overtraining significantly inhibited phagocytosis of MФs （declined by 27%,P〈0. 05）. When compared with the control group, IGF-1 and MGF mRNA level in MФs from overtraining group increased 21-fold and 92 fold, respectively （P〈0.01）. In vitro experiments showed that IGF-1 had no significant effects on phagocytosis of MФs, there were no significant difference in each concentration when compared with untreated cells（P〉0.05）. Unlike IGF 1, MGF peptide impaired the phagocytosis of MФs in dose-independent manner. There were signifcant differences in 10 ng/ml （P〈0.05）, 50 ng/ml （P〈0.05）, 100 ng/ml （ P〈 0.01 ）, 200 ng/ml （ P〈 0.01 ）, when compared with the untreated cells. There were no significant difference between different concentrations of MGF （P〈0. 05）. Conclusion：These results suggest that overtraining inhibits the phagocytosis of p