中文摘要：

癌症的攻克和预防需要更为灵敏和特异的早期诊断和术后控制标志物．基于生物质谱的定量蛋白质组学技术由于灵敏度高、分析速度快、通量高，在肿瘤标志物的筛查中得到了广泛的应用．终端等重同位素标记串级质谱定量方法，通过对肽段的N端和C端进行互补的同位素标记产生等重标记的肽段，在一级质谱中呈现相同的质荷比，而在串级质谱中产生碎片离子对用于蛋白质的定性和定量．该方法以其简单的操作、高准确度和高通量的优势为生物标志物的发现提供了多种新的思路，为肿瘤标志物筛查提供新的手段．本文综述了终端等重同位素标记串级质谱定量方法的最新发展及其在生物标志物研究中的应用．

英文摘要：

More specific and sensitive biomarkers in early diagnosis and prognosis are required for curing and preventing cancer. Quantitative proteomics methods are widely used in biomarker discovery because of high accuracy, sensitivity and specificity. In the isobaric terminal labeling quantification, peptide terminis are separately derivatized with complementary isotopically labeled reagents. As a result, the peptide gain the same molecular weight in the MS spectrum, and all N- and C-terminal fragment ions will provide quantification data for each peptide throughout the MS/MS spectrum. Because of the simple operation steps, high accuracy and high throughput, such method offers a wide range of ideas for biomarker discovery and screening. We reviewed here all current developed isobaric terminal labeling quantification techniques and their applications in cancer biomarker research.