中文摘要：

目的 探讨胃泌素对结肠癌细胞Colo320WT中β—catenin／Tcf-4通路影响。方法10^-8mol·L^-1胃泌素分别于0、1、6、12、24、48h时间点干预Colo320WT细胞，同时先用10^-6mol·L^-1胃泌素受体拮抗剂1365，260预干预Colo320WT细胞30min，再加10^-8mol·L^-1胃泌素干预至12h。采用免疫共沉淀和免疫印迹检测Colo320WT中TX-100可溶性部分（胞质部分）和不可溶部分（细胞骨架结合部分）中β-catenin的表达及β-catenin／Tcf-4复合物的表达。免疫印迹观察c—Myc和cyclinD1的表达。结果TX-100可溶性部分中β—catenin的量随着胃泌素干预时间呈递减趋势，12h达最小；而TX-100不溶解部分却随时干预时间的呈递增趋势，12h达最大。免疫共沉淀发现胃泌素干预后β-catenin／Tcf-4复合物表达量明显增多。免疫印迹检测到胃泌素干预下的细胞中c—Myc和cyclinD1表达明显增加。胃泌素受体拮抗剂阻断后，则可阻断胃泌素引起的上述效应。结论胃泌素与其受体作用后使结肠癌细胞中β-catenin分布发生转移和活化β-catenin／Tcf-4通路来降低结肠癌细胞的黏附，增加肿瘤细胞的侵袭和转移。

英文摘要：

Aim To explore the effect of gastrinl7 on expression of β-catenin/Tcf-4 pathway in colonic carcinoma cell line Colo320WT. Methods Colo320WT cells were treated with 10^-8 mol· L^-1 gastrin17 in time-dependent way （ 0.1.6.12.24.48 h ）, while treated with 10^-6 mol· L^-1 L365,260（ gastrin17 receptor blocker） for 30 minutes and treated with gastrin17 again for 12 h. Theexpression levels of β-catenin in TX-100 solution fraction（cytoplasmic） and TX- 100 insolution fraction （cytoskeleton bound ） of Colo320WT cells, and the expression levels of β-catenin/ Tcf-4 complex were detected by coimmunoprecipation and Western blot. The expression levels of c-Myc and cyclinD1 were assayed by Western blot. Results Expression levels of β-catenin in TX-100 solution fraction were decreased apparently, but increased again by L365,260 blocking. Expression levels of 15-catenin in TX-100 insolution fraction were on the contrary. Expression levels of β-catenin/Tcf-4 complex increased apparently. Expression levels of c-Myc and cyclinD1 in Colo320WT treated by gastrin17 were higher markedly than those of Colo320WT untreated and treated by L365,260. Conclusions Gastrinl7 interacted with CCK-2R and effected significantly distribution of β- catenin in Colo320WT and activated β-catenin/Tcf-4 pathway which led to c-Myc and cyclinD1 high expression,the way gastrin17 decreased cell-cell cohesion and increased tumor cells invasion and metastasis further.