中文摘要：

MaMV-DC cyanophage，感染形成花蕾的 cyanobacterium Microcystis aeruginosa，从达恩奇湖，被孤立 Kunming，中国。21 cyanobacterial 紧张被用来检测 MaMV-DC 的主人范围。Microcystic aeruginosa FACHB-524 和匾纯化被用来孤立单个 cyanophages，并且有 cyanobacteria 的 culturing MaMV-DC 允许我们为进一步的分析准备净化的 cyanophages。电子显微镜学证明否定地染色的病毒的粒子近似与一个 icosahedral 头一起是塑造蝌蚪的在直径和一条可收缩的尾巴的 70 nm 在长度的约 160 nm。用一步舞生长实验， MaMV-DC 的潜伏期和爆炸尺寸被估计分别地每房间是 2448 个小时和约 80 个传染单位。限制 endonuclease 消化和 agarose 胶化电气泳动用净化的 MaMV-DC genomic DNA，和染色体尺寸被执行被估计是约 160 kb。钠 dodecyl 硫酸盐 polyacrylamide 胶化电气泳动(SDS 页) 分析揭示了四主要结构的蛋白质。这些结果在使用淡水 cyanophages 控制形成花蕾的 cyanobacterium 支持成长兴趣。

英文摘要：

The MaMV-DC cyanophage, which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi, Kunming, China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages, and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments, the latent period and burst size of MaMV-DC were estimated to be 2448 hours and approximately 80 infectious units per cell, respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA, and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.