中文摘要：

目的通过评价线粒体功能研究线粒体介导的caspase-9激活通路参与成人大骨节病关节软骨细胞凋亡的机制。方法关节软骨细胞培养,Hoechst 33258染色检测软骨细胞核形态,流式细胞仪检测软骨细胞凋亡率和细胞内活性氧含量,Western bloting检测细胞色素c的表达,荧光法检测caspase-9和caspase-3活性。结果大骨节病患者关节软骨细胞较正常软骨细胞凋亡百分率增加,线粒体细胞色素c释放,caspase-9和caspase-3活性增高,且活性氧含量增加。结论线粒体氧化应激参与了大骨节病软骨细胞的病理生理变化,线粒体功能障碍可能在大骨节病关节软骨细胞凋亡过程发挥了重要作用。

英文摘要：

Objective To evaluate the mitochondrial function to understand the mitochondria-mediated caspase-9 activation and apoptosis in adult Kashin-Beck disease（KBD） chondrocytes.Methods Apoptotic cell was evaluated by analyzing the apoptosis rate of KBD articular chondrocytes and the caspase-9 and 3 activities.Mitochondrial function was evaluated by analyzing the cytochrome c release from mitochondria to the cytosol and the intracellular reactive oxygen species（ROS） contents.Results The percentage of positive apoptotic chondrocytes from the KBD patient group exhibited a higher level than that of the healthy controls.Mitochondrial cytochrome c release and caspase-9 and 3 activation were also found.Conclusion These findings suggest the involvement of mitochondrial oxidative stress and celluar apoptosis in the pathophysiology of KBD.The dysfunction of mitochondria may play an important role in KBD articular chondrocytes apoptosis.