中文摘要：

目的探讨脱氢穿心莲内酯琥珀酸半酯（DAS）对于急性肺损伤状态下水通道蛋白5（AQP-5）的调节作用及对肺水肿清除的影响。方法30只雄性BALB／C小鼠平均分为对照组（Control组）、治疗组（LPS＋DAS组）和内毒素组（LPS模型组），每组10只。通过HE染色观察肺组织病理学改变；使用湿／干比（w／D）分析肺水肿程度；使用RT-PCR和westernblotting对AOP-5的表达进行测量。结果小鼠气道内注射内毒素（LPS）72小时后肺损伤评分和w／D比值明显增加，AQP-5表达量水平明显下降，差异有显著统计学意义（均P〈O．05）。同时发现，LPS＋DAS组小鼠肺损伤评分及w／D比值较LPS组较低，而AQP_5表达量较之升高，差异均有统计学意义（均P〈0．05）。结论脱氢穿心莲内酯琥珀酸半酯可以有效减轻LPS诱导的小鼠ALI导致的肺水肿，其机制可能与上调了AQP-5的表达相关。该结果为脱氢穿心莲内酯琥珀酸半酯治疗ALI／ARDS相关的肺水肿奠定了实验基础。

英文摘要：

Objective To investigate the therapeutic value of dehydroandrographolide succinate （DAS） on the ex- pression of AQP-5 in LPS-induced acute lung injury in mice. Methods Thirty male BALB/C mice were randomly divided into 3 groups, Control group, LPS-FDAS group and LPS group （n= 10）. HE staining and lung injury score system were used to evaluate the pathological changes in lung. Wet to dry ratio （W/D） was included to measure the degree of lung e- dema. RT-PCR and western blotting were used to measure AQP-5 expression. Results After 72h LPS stimulation, se- vere pathological changes, increased W/D and down-regulated AQP-5 expression were observed. However, compared with LPS group, pathological changes and W/D were significantly ameliorated, meanwhile, AQP-5 expression was markedly up-regulated was notably reduced in LPS-I-DAS group. Conclusion Our results indicated that DAS attenuates oulmonarv edema in LPS-indueed acute lung injury possibly through up-regulation of aquaporin-5.