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奶水牛乳汁体细胞数与抗氧化指标的相关性分析
  • ISSN号:2095-1191
  • 期刊名称:《南方农业学报》
  • 时间:0
  • 分类:S823.83[农业科学—畜牧学;农业科学—畜牧兽医]
  • 作者机构:[1]广西大学动物科学技术学院,南宁530005
  • 相关基金:国家自然科学基金项目(31260631);广西大学大学生创新计划项目(SYJN20131722)
中文摘要:

[目的]了解奶水牛乳汁体细胞计数(SCC)与抗氧化指标之间的相关性,为乳汁质量监控和奶水牛健康养殖提供科学依据.[方法]随机选取南宁某牛场奶水牛139头,同步采集乳样与血样,乳汁SCC采用FOSS体细胞仪测定,血浆和乳清的总抗氧化能力(TAC)采用FRAP法(血浆铁还原力法)测定,丙二醛(MDA)含量采用TBA法(硫酸巴比妥法)测定.[结果]被检的139头奶水牛中,乳汁SCC≥50.00万/mL(隐性乳房炎诊断标准)的样本占被检总数的12.23%,乳汁SCC<50.00万/mL的样本占被检总数的87.77%;奶水牛血浆和乳清的TAC分别为2.44±1.04和3.78±1.68 U/mL,MDA含量分别为2.07±0.48和1.55±0.52 nmol/mL,其差异均达极显著水平(P<0.01).乳清TAC随乳汁SCC的升高而呈下降趋势,二者间呈显著负相关(r=-0.168,P<0.05);血浆MDA与乳清MDA间呈显著正相关(r=0.189,P<0.05),而血浆MDA与血浆TAC(r=0.391)、乳清MDA与血浆TAC(r=0.315)间呈极显著正相关(P<0.01).[结论]乳清TAC可作为检测奶水牛乳房健康及其乳汁质量的备选指标.

英文摘要:

[Objective]The correlationship between somatic cell count (SCC) and antioxidant parameters was analyzed so as to provide scientific references for milk quality monitoring and healthy breeding of dairy buffalo.[Method]A total of 139 dairy buffalo cows were randomly chosen from one buffalo farm in Nanning.Milk and blood samples of each buffalo were collected simultaneously.Milk SCC was analyzed by FOSS instrument,and the serum and whey contents of total antioxidant capacity(TAC) and malondialdehyde (MDA) were detemined using Ferric Reducing Ability of Serum(FRAP) method and Thiobarbituric acid (TBA) method,respectively.[Result]Out of the tested 139 buffaloes,12.23% of buffalo cows had milk SCC ≥50.00×104/mL (as diagnostic criterion for subclinical mastitis),the remainder 87.77% of buffalo had SCC < 50.00× 104/mL.Serum and milk content of TAC were 2.44±1.04 and 3.78±1.68 U/mL,respectively.Those of MDA were 2.07±0.48 and 1.55±0.52 nmol/mL,respectively.Both TAC and MDA had very significant differences between blood and milk (P<0.01).Milk serum content of TAC clearly decreased with milk SCC increase.A significant negative correlation existed between milk TAC content and milk SCC(r=-0.168,P<0.05).There was a significant positive correlation between blood MDA and milk MDA(r=0.189,P<0.05).An extraordinary significant positive correlation was found between blood TAC and blood MDA (r=0.391,P<0.01),and also between blood TAC and milk MDA (r=0.315,P<0.01).[Conclusion]Milk TAC content can be used as an alternative parameter for monitoring buffalo udder health and milk quality in future.

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期刊信息
  • 《南方农业学报》
  • 中国科技核心期刊
  • 主管单位:广西壮族自治区农业科学院
  • 主办单位:广西壮族自治区农业科学院
  • 主编:李杨瑞
  • 地址:南宁市大学东路174号
  • 邮编:530007
  • 邮箱:nfnyxb@163.com
  • 电话:0771-3240518
  • 国际标准刊号:ISSN:2095-1191
  • 国内统一刊号:ISSN:45-1381/S
  • 邮发代号:48-3
  • 获奖情况:
  • 第六届广西十佳自然科学期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘
  • 被引量:5649