中文摘要：

目的：研究IFN—α抗HCV的作用机理及了解ISG20是否参与介导IFN-α对HCV的抑制作用。方法：用RT—PCR法及融合PCR法分别获得野生型及突变型ISG20cDNA，并将其克隆到真该表达载体pcDNA3．1上，转染含HCV复制子的Huh7细胞进行瞬时表达，通过Northern blot及Western blot分别检测HCVRNA及NS5A蛋白水平，研究表达ISG20对HCV复制子的影响。结果：构建的野生型及突变型ISG20真核表达载体在mRNA水平及蛋白水平的表达均得到证实，并且发现表达野生型ISG20对HCV复制子RNA有抑制作用。结论：成功克隆及表达了ISG20，并对其抗病毒作用进行了初步研究，提示ISG20可能介导IFN-α对HCV的抑制作用。

英文摘要：

Objective：To study the antiviral effect of ISG20 on HCV replicon. Methods：Wild type ISG20/mutated ISG20 cDNAs were obtained by RT-PCR/two step-PCR directed mutagenesis, and wild type ISG20 and dominant negative mutated ISG20 mammal expression vectors were constructed. The constructed pISG20wt and pISG20m expressing vectors were transfected into Huh7 cells or Huh7 ceils containing HCV replicon to investigate its effects on HCV replicon replication. Results：The ISG20wt/ISG20m expression vectors were constructed and the expressions of these two vectors were confirmed at both mRNA and protein levels. The effects of ISG20wt on HCV replicon replication were evaluated by Northern blot and Western blot. The results showed that expression of ISG20wt had significant inhibitory effect on HCV RNA replication. Conclusion ： ISG20 participates in the anti-HCV action of IFN-α on HCV replicon system.