应用光学显微镜、紫外分光光度计和傅立叶显微红外光谱技术,对不同CO2浓度(370、700μmol·mol^-1)处理条件下,对拟南芥茎秆的形态结构及化学成分进行解剖学和组织化学的对比研究.结果发现,在CO2浓度升高后,茎秆的直径、面积、形成层细胞活性及维管束面积等均有所增加;化学成分分析显示,茎秆中纤维素、木质素以及可溶性糖的含量均比对照有所提高,且纤维素和可溶性糖增加显著.研究表明,拟南芥茎秆的解剖结构及化学成分对大气CO2浓度升高表现出正响应,从而为提高茎秆水分和营养物质的运输以及对生殖器官的支撑等各项功能提供更多的结构和物质基础.
Arabidopsis thaliana were grown under ambient (370μmool·mol^-1) CO2 and elevated (700μmool·mol^-1) CO2 ,respectively. In the present study,the floral stems were sampled for the investigation of the effects of elevated CO2 concentration on the morphological traits,anatomical features and chemical composition of the floral stems using light microscopy and Fourier transform infrared spectroscopy. It was found that elevated CO2 treatment caused significant increase on anatomical traits of the floral stems of A. thaliana, including stem size, cambium activity as well as vascular bundles area. In addition, the results of chemical composition analysis revealed that elevated CO2 treatment enhanced the content of cellulose,lignin and total soluble sugar in comparison with ambient CO2 treatment, especially cellulose and lignin. These results suggested that the floral stems of A. thaliana respond positively to elevated CO2 concentration. They have provided more structural and material foundations for the transportation of nutrients and water,to support reproductive organ.