采用液体培养研究Pb、NaCl单因子及复合胁迫对喜盐鸢尾(Iris halophilaPall.)的生长和生理变化的影响以及Pb在鸢尾植株不同部位的积累.结果显示,培养8 d后,随着Pb+NaCl复合浓度的增加,喜盐鸢尾地上部和地下部干重下降;当Pb处理浓度提高,喜盐鸢尾地上部和地下部Pb含量显著增加,在5 mmol·L-1Pb+50mmol·L^-1NaCl胁迫下,地上部Pb含量达到1 534μg·g^-1,达到超富集植物的标准,但植株干重下降不显著;NaCl对Pb的吸收有一定促进作用.Pb、NaCl单因子或复合胁迫均增加了对喜盐鸢尾幼苗的伤害,在100 mmol·L^-1NaCl及Pb和NaCl复合胁迫下,MDA含量和Pro含量显著增加,在10 mmol·L^-1Pb、100 mmol·L^-1NaCl及Pb和NaCl复合胁迫下SOD活性显著提高.结果表明,喜盐鸢尾具有一定的耐Pb和NaCl胁迫的能力,并对Pb具有潜在的超富集能力,是一种可用于修复Pb污染环境的Pb潜在超富集植物.
The effects of Pb,NaCl and Pb NaCl interaction on the plant vigor,Pb accumulation in different parts of plants and physiological mechanisms of Iris halophila Pall. were investigated in hydroponic system. The dry weights (DW) of shoots and roots decreased as the increase of Pb+NaCl concentration after 8 days exposure. The Pb contents increased significantly with Pb higher concentration treatment. The Ph content was 1 534μg·g^-1 DWat 5 mmol·L^-1 Pb+50 mmol·L^-1 NaCl treatment and the DW did not drop significantly. The Pb accumulation could be enhanced by NaCl. The contents of malondialdehyde (MDA) and proline (Pro) significantly increased at the treatments of 100 mmol·L^-1 NaCl and Pb+NaCl stresses. The activities of superoxide dismutases (SOD) significantly increased at the levels of 10 mmol·L^-1 Pb, 100 mmol·L^-1 NaCl and Pb+NaCl stress. The results showed that I.halophila could be tolerant to NaCl and Pb stress,at least up to 100 mmol·L^-1 NaCl and 5 mmol·L^-1 Pb,and was a potential Pb hyperaccumulator for remediation of Pb polluted environment.