中文摘要：

目的：探讨4种生长因子对体外培养的人牙乳头细胞中核心结合因子a1（core binding factor al，cbfal）表达的影响。方法：体外培养人牙乳头细胞，转染pcDNA3-cbfal重组质粒，G418筛选建立稳定表达cbfal的细胞模型PC-3，分别在正常细胞和PC-3细胞中加入不同浓度的BMP-2、TGF-β1、FGF-2、EGF，采用RT-PCR、Western blot、免疫组织化学的方法，观察这4种生长因子在人牙乳头细胞中对cbfal基因表达、蛋白合成、蛋白表达部位的影响。结果：200ng／mLBMP-2和50ng／mL的FGF-2刺激正常人牙乳头细胞6h后cbfal的表达明显升高，而20和40ng／mL的TGF-β1作用12h，能抑制稳定转染cbfal基因的细胞中cbfal mRNA的表达；EGF则对人牙乳头细胞中cbfal的表达没有显著影响。结论：BMP-2和FGF-2可以上调人牙乳头细胞中cbfal的表达，TGF-β1对cbfal的表达有抑制作用，EGF则对人牙乳头细胞中cbfal的表达没有显著影响。

英文摘要：

AIM ：To explicit whether the expression of cbfal is regulated by BMP -2, TGF - β1, FGF -2 and EGF in human dental papilla cells, METHODS：Human dental papilla cells were cultured in vitro and transfected with pcDNA3 -cbfal recombinant plasmids. After selected with G418 sulfate, a cell clone named PC -3, which could stably express the cbfal mRNA and protein,was proved by PCR and western blot, Then the expression of cbfal in normal cells and PC -3 cells treated with the four growth factors were detected by immunohistochemistry, western blot and PCR methods, RESULTS： The expression of cbfal were obviously upregulated in normal cells after 6 h treated with 200 ng/mL BMP- 2 or 50ng/mL FGF -2, and its in PC -3 cells was downregulated after 12 h treated with 20 or 40 ng/mL TGF - β1. EGF has no effect on the expression of cbfal in cells, CONCLUSION： In human dental papilla cells,cbfal could be upregulated by BMP- 2 or FGF -2 ,and downregulated by TGF- β1,