中文摘要：

目的探讨人参皂苷Rg1对脂多糖（LPS）诱导的原代星形胶质细胞及BV2小胶质细胞炎症反应的抑制作用及糖皮质激素受体（GR）阻断剂RU486对其的影响。方法常规培养原代星形胶质细胞及BV2小胶质细胞,随机分为对照组、LPS组、Rg1＋LPS组、RU486＋Rg1＋LPS组。对照组不做任何特殊处理,其余各组在有或无Rg1预处理条件下,首先加入RU486（1μmol/L）作用1h,继以1mg/L的LPS共同作用细胞6h。应用实时反转录聚合酶链式反应检测各组诱导型一氧化氮合酶（iNOS）基因的表达。结果与对照组比较,加入1mg/L的LPS能明显上调星形胶质细胞和BV2小胶质细胞iNOS基因的表达（F=82.69、18.73,q=21.530、8.974,P〈0.01）。与LPS组相比较,加入Rg1能明显抑制LPS对星形胶质细胞和BV2小胶质细胞iNOS的诱导作用（q=9.799、6.640,P〈0.01）;RU486＋Rg1＋LPS组与Rg1＋LPS组iNOS基因表达比较,差异无统计学意义（P〉0.05）。结论人参皂苷Rg1能够抑制LPS诱导的原代星形胶质细胞及BV2小胶质细胞iNOS的基因表达,此作用不能被GR特异性阻断剂RU486所阻断。

英文摘要：

Objective To investigate the inhibitory effect of ginsenoside Rg1 on lipopolysaccharide（LPS）-induced inflammatory response in primary cultured astrocytes and BV2 microglial cells and the influence of glucocorticoid receptor（GR）antagonist RU486. Methods Primary astrocytes and BV2 microglial cells were cultured and randomly divided into control group,LPS group,Rg1＋LPS group,and RU486＋Rg1＋LPS group.No treatment was given to the cells in the control group;the cells in the other three groups were treated with RU486（1μmol/L）for 1hin the presence or absence of Rg1 pretreatment,followed by1mg/L LPS for 6h.Real-time reverse transcription-polymerase chain reaction was used to measure the mRNA expression of inducible nitric oxide synthase（iNOS）. Results Compared with the control group,the LPS group had a significant increase in the mRNA expression of iNOSin astrocytes（F=82.69,q=21.53,P〈0.01）and BV2 microglial cells（F=18.73,q=8.974,P〈0.01）.Compared with the LPS group,the Rg1＋LPS group showed significant inhibition of the inductive effect of LPS on iNOSin astrocytes and BV2 microglial cells（q=9.799,6.640;P〈0.01）.There was no significant difference in the mRNA expression of iNOSbetween the RU486＋Rg1＋LPS group and the Rg1＋LPS group（P〉0.05）. Conclusion Ginsenoside Rg1 can inhibit LPS-induced mRNA expression of iNOSin astrocytes and BV2 microglial cells.This effect cannot be blocked by the GR antagonist RU486.