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基于线粒体细胞色素b基因序列分析的泉水鱼遗传多样性研究
  • ISSN号:1004-874X
  • 期刊名称:《广东农业科学》
  • 时间:0
  • 分类:Q343.35[生物学—遗传学] Q346.5[生物学—遗传学]
  • 作者机构:[1]暨南大学水生生物研究所.广东广州510632
  • 相关基金:国家自然科学基金(30770415,41071034);暨南大掣科研培育与创新基金(21609710,21611426)
中文摘要:

测定了长江水系贵定和乐山2个群体30尾泉水鱼细胞色素b基因1 026 bp序列,发现13个变异位点,检测出7种单倍型,Hd和Pi分别为0.772和0.0041,其中贵定和乐山群体的Hd分别为0.695和0.362,Pi分别为0.0030和0.0004,呈现出单倍型多样性较低和核苷酸多样性极低的特点;两个群体间的Fst和Nm值分别为0.7417和0.17,AMOVA分析显示遗传变异主要集中在群体间(74.17%),表明群体间出现高度的遗传分化;在邻接树上出现两个按地理来源聚群谱系,呈现出明显的地理结构;估算群体间分歧时间大约在16万年前,为更新世时期。中性检测表明两个群体在过去没有发生种群的快速扩张。建议将贵定和乐山群体作为不同的管理保护单位加以保护。

英文摘要:

Abstract: 1 026 bp sequences of mitochondrial DNA cytochrome b gene of 30 individuals from 2 populations of Pseudogrincheilus procheiluys collected in Guiding and Leshan from the Yangtze River drainage respectively were sequenced, totally 13 variable sites were found in 7 baplotypes, the global haplotype diversity (Hd) and nucleotide diversity was 0.772 and 0.0041 respectively, the haplotype diverisity in Guiding and Leshan populations were 0.695 and 0.362 respectively, their nueleotidc diversity were 0.0030 and 0.0004 respectively, indicating a pattern of low haplotype diversity and very low nucleotide diversity. Fst and Nm values between the two populations were 0.7417 and 0.17 respectively, and 74.17% genetic variation occurred between populations in AMOVA analysis, suggesting they were highly differentiated. Obvious geographic structure were revealed by 2 lineages coincided with geographic clusters formed in the Neighbor-joining tree, and the estimated divergence time were about 160 000 years ago, i.e., in the Holocene. No rapid expanding was found by using the neutrality tests, and the two populations from the Yangtze River should be protected as two different management units.

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期刊信息
  • 《广东农业科学》
  • 北大核心期刊(2011版)
  • 主管单位:广东省农业科学院
  • 主办单位:广东省农业科学院 华南农业大学
  • 主编:
  • 地址:广州市天河区金颖路31号五山广东省农科院农经所
  • 邮编:510640
  • 邮箱:gdnykx@vip.163.com
  • 电话:020-38319948 38319941 38319942 38319946
  • 国际标准刊号:ISSN:1004-874X
  • 国内统一刊号:ISSN:44-1267/S
  • 邮发代号:
  • 获奖情况:
  • 广东省第二、第三届优秀科技期刊,广东省首届十佳期刊,中国期刊方阵“双效”期刊,第四届全国农业优秀期刊一等奖,第五届全车优秀农业期刊鑫犁奖一等奖,首届全国CAD规范一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国动物学记录,中国北大核心期刊(2008版),中国北大核心期刊(2011版)
  • 被引量:33995