利用来自中豆29x中豆32的165个重组自交系F10进行2年田间试验,以复合区间作图法检测与大豆倒伏及形态性状有关的QTL。结果表明,2年分别检测到25个和19个与大豆倒伏及茎杆性状和根系性状有关的QTL,分布于A2、C1、C2、D1a、F、G、I和L连锁群,可解释4.4%~50.1%的表型变异。在F连锁群上,2年均检测到倒伏主效QTL(qLD-15-J)和株高主效QTL(qPH-15—2);G连锁群有1个主茎节数QTL、A2和L连锁群上分别有1个根重QTL在2个年度重复出现。在倒伏QTL的附近检测出株高、根重、茎叶重、茎粗、主茎节数和分枝数QTL,表明植株地上部和地下部性状与抗倒性普遍关联;QTL定位结果与表型相关分析一致,反映了这些形态性状表型相关的遗传特性。部分性状QTL存在共位性,但是未在2个年度稳定表达。
Lodging is an important agronomical trait in soybean, which has obvious negative effect on yield, seed quality and mechanical harvesting efficiency. The 165 recombinant inbred lines (RIL) derived from a cross between Zhongdou 29 and Zhongdou 32 were used in two years, with the composite interval mapping (CIM) method to identify the QTLs associated with lodging, plant height, stem diameter, nodes on main stem, branch number, shoot weight, root weight and stem intension. On linkage groups A2, C1, C2, D1a, F, G, I, and L, 25 and 19 QTLs for lodging and related traits were detected in 2006 and 2007, respectively, explaining 4.4-50.1% phenotypic variation. One QTL (qLD-15-1) of lodging and one QTL (qPH-15-2) of plant height on F linkage group were both detected in two years; one QTL of nodes on main stem and two QTLs of root weight were detected in two years on G, A2, and L linkage groups respectively. The positions of some QTLs of plant height, root weight, shoot weight, stem diameter, nodes on main stem and branch number were close to those of lodging, showing that the lodging resistance is associated with the characters in aerial part and underground portion, the results were consistent with the correlation analysis of phenotypic traits, showing the correlation of these morphological traits in genetics. The QTLs of some traits had the same positions, but were not both detected in two years.