将促藻菌株微小杆菌(Exiguobacterium sp.)013(E.sp013)和芽孢杆菌(Bacillus sp.)D06(B.spD06)复壮后与铜绿微囊藻(Microcystis aeruginosa)、水华微囊藻(Microcystis flos-aquae)和惠氏微囊藻(Microcystis wesenbergii)的休眠体经底泥浸润包埋,进行底泥原位模拟试验,对比分析底泥表层2 个菌株对3 种微藻休眠体复苏的影响.结果表明:在E.sp013 作用下,铜绿微囊藻、水华微囊藻和惠氏微囊藻休眠体复苏的最低温度均降低1 ℃(对照组分别为11、12 和12 ℃),与对照组均存在显著差异(独立样本t 检验,P〈0?? 05),而B.spD06 并无此现象,但能提高3 种微藻休眠体复苏率.同一条件下,E.sp013 促3 种微囊藻休眠体复苏强度强于B.spD06.E.sp013 和B.spD06 均能使微囊藻休眠体复苏时间提前,E.sp013 和B.spD06 对铜绿微囊藻、水华微囊藻和惠氏微囊藻休眠体复苏时间均提前约1 d,2 个菌株在促3 种微囊藻休眠体复苏时间的提前上并无显著差异(Duncan 多范围检验方法,P〉0. 05).E.sp013 和B.spD06 通过自身对营养元素N 的利用和除N 能力降低了水体中的N/ P 比,这可能是促进3 种微囊藻休眠体复苏的原因.
An in?situ simulation experiment was conducted embedding rejuvenated algae?promoting strains of bacteria, Ex?iguobacterium sp. 013 and Bacillus sp. D06, separately, with hypopuses of Microcystis aeruginosa, Microcystis flos?aqua and Microcystis wesenbergii in wet sediment to compare effects of the formers on anabiosis of the latters. Results show that as affected by E.sp013 the hypopuses of Microcystis aeruginosa, Microcystis flos?aqua and Microcystis wesenbergii started to resuscitate at a temperature 1 ℃ lower than that in their control groups, respectively,( 11, 12 and 12℃, respectively) , while B.spD06 did not have such an effect, but it did increase resuscitation rates of the hypopuses. Under the same condi?tion, E.sp013 was stronger than B.spD06 in effect of promoting resuscitation of the hypopuses. But both of them advanced resuscitation of the hypopuses in time by one day. No significant difference was found between the two strains of bacteria and between the three kinds of Microcystis in this effect ( Duncans multiple range test, P>0?05) . The experiment also re?veals that E.sp013 and B.spD06 bacteria reduced N/P ratio in the waterbody through their ability to assimilate N nutrient, thus removing N from the waterbody, which may be the reasons why the 2 strains of bacteria can promote resuscitation of the hypopuses of the 3 kinds of Microcystis.