通过设置缺硫(S)处理,研究了镉(Cd)胁迫下水稻生长情况、幼苗Cd和非蛋白巯基含量以及谷胱甘肽硫转移酶(GST)活性的动态变化.结果表明:Cd胁迫明显抑制了水稻生长,显著诱导了巯基物质[非蛋白巯基(NPT)、谷胱甘肽(GSH)、植物螯合肽(PC)]的合成,GST活性表现出先升后降的趋势.缺S处理下,尽管水稻根部对Cd的吸收和向地上部的转运都有所增加,但Cd胁迫程度并未明显增强,巯基物质含量明显降低,根部GST活性提高.表明巯基物质和GST在水稻抗Cd胁迫过程中互为补充,在一定程度上减轻了Cd的毒性效应.
By installing a treatment of sulfur starvation,this paper studied the dynamic changes of rice seedling growth,cadmium(Cd) and non-protein thiol(NPT) contents,and glutathione S-transferase(GST) activity under Cd stress.Cd stress inhibited the seedling growth obviously,induced the synthesis of sulfhydryl(-SH) compounds,including non-protein thiol,glutathione,and phytochelatins,and made the GST activity decreased after an initial increase.Sulfur starvation somewhat increased the Cd uptake and translocation,but less affected the impacts of Cd stress.The contents of-SH compounds decreased,and the GST activity in root increased.It was suggested that the roles of-SH compounds and GST in Cd-resistance of rice were complementary,being able to alleviate the Cd toxicity to some extent.