中文摘要：

利用猪流感病毒（Swine influenza virus，siv）与伪狂犬病病毒（Pseudorabies virus，PRV）血清学阴性的4周龄断奶仔猪，对此前构建的表达SIVA／Swine／Inner Mogolian／547／2001（H3N2）血凝素（HA）基因的重组伪狂犬病病毒（rPRV-HA）进行了免疫效力评价。按每头10^5.0 PFu rPRV-HA肌肉注射断奶仔猪（n=12），同时设Bartha-K61免疫对照组（n=5）和非免疫攻毒对照组（n=5）。免疫后35d用2×10^5.0 TCID50的SIVA／Swine／Heilongjiang／74／2000（H3N2）进行强毒攻击。rPRV-HA免疫组免疫后7d均可检测到高滴度针对SIV的血凝抑制（HI）抗体，而所有对照组小猪仅在攻毒后7d才开始产生针对SIV的HI抗体；rPRV-HA免疫组在病理学保护方面明显优于对照组，支气管和肺泡冲洗液病毒分离结果显示，攻毒后7drPRV-HA免疫组没有检测到病毒，而两个对照组可检测到病毒。以上试验结果表明，rPRV-HA免疫猪对同亚型SIV的攻击具有很好的保护作用。

英文摘要：

A recombinant pseudorabies virus （rPRV-HA） expressing the hemagglutinin （HA） gene of swine influenza virus （SIV） A/Swine/Inner Mogolian/547/2001 （H3N2） was immunologically evaluated in piglets. Twelve 4-weeks old healthy piglets were inoculated intramuscularly （i.m.） with 105.0 PFU of rPRV-HA. Two control groups （five piglets each） were either mock-inoculated or inoculated with PRV Bartha-K61. Piglets were challenged intranasally with 2 × 10^5.0 TCID50 of A/Swine /Heilongjiang/74/2000 （H3N2） （SwHLJ74） at 35 days post-inoculation. Serological detection showed that piglets inoculated with rPRV-HA developed a high level of virus-specific hemagglutination-inhibition （HI） antibody at seven days post vaccination. In comparison, pigs in the two control groups only developed virus-specific HI antibody at seven days post challenge. Anti-PRV neutralizing antibodies were detected at seven days post vaccination in the sera of all piglets inoculated with rPRV-HA and Bartha-K61. Piglets immunized with rPRV-HA were shown protected from histopathological effects. Compared to the control group, SIV in bronchus and lung lavage fluids of rPRV-HA immunized pigs could be detected for a much shorter time after challenge. Thus, the rPRV-HA can be a potential vaccine candidate for preventing the same subtype of SIV infection.