中文摘要：

本试验建立了中蔬四号番茄的再生频率高、所需时间短、试管苗状态好的高效再生体系。具体培养流程如下：以下胚轴为外植体，在Murashige和Skoog（MS）＋1．0mg几玉米素（ZT）＋0．1mg／L 3-吲哚乙酸（IAA）或MS＋2．0mg/L 6-苄基腺嘌呤（BA）＋0．2mg／L IAA上进行不定芽诱导培养21天后，继代一次，转接到MS＋0．1mg／L IAA中进行生根：炼苗2～3天，转栽至蛭石中，最后移栽到菜园土中培养。

英文摘要：

A high efficient tomato （Solanum lycopersieum L. cv Zhongshu No. 4） regeneration system with high frequency regeneration ratio, time-saving and good tube seedlings was developed. Detailed protocols were as follows： Hypocotyls were placed on Murashige and Skoog （MS） medium supplemented with 1.0 mg/L zeatin （ZT） and 0.1 mg/L indole-3-acetic acid （IAA） or 2.0 mg/L 6-benzyladenine （BA） and 0.2 mg/L IAA for inducing adventitious buds; after being cultured for 21 d, hypocotyls were sub-cultured to fresh medium once, and then the buds were cut and induced roots on MS medium supplemented with 0.1 mg/L IAA; well-rooting buds were acclimated to greenhouse conditions for 2-3 d, and then transplanted to well-watered vermiculite for 3-7 d, finally transplanted to vegetable garden soil.