[目的]为了获得虫龄整齐、生理状态一致的幼虫及虫体内无松材线虫的成虫,开展了松墨天牛的室内人工饲育技术研究。[方法]利用自行配制的人工饲料3种配方:A(松树木屑100 g+琼脂40 g+蔗糖20 g+酵母粉125 g+安息香酸钠2 g+山梨酸钾1 g+05 mol/L H2SO410 mL+水800 mL)+B(松树木屑100 g+琼脂40 g+蔗糖20 g+酵母粉125 g+安息香酸钠2 g+山梨酸钾1 g+05 mol/L H2SO410 mL+胆固醇15 g+抗坏血酸4 g+酪蛋白20 g+氯化胆碱1 g+水800 mL)+C(松树木屑100 g+琼脂40 g+蔗糖20 g+酵母粉125 g+安息香酸钠2 g+山梨酸钾1 g+05 mol/L H2SO410 mL+麦胚粉25 g+胆固醇15 g+抗坏血酸4 g+酪蛋白20 g+氯化胆碱1 g+水800 mL),比较了人工饲料配方对松墨天牛发育历期及生活力的影响。[结果]A、B、C 3种人工饲料饲养的松墨天牛幼虫均可正常生长发育。发现取食配方A的幼虫发育历期平均为(906±93)d,蛹期为(119±13)d,化蛹率为64%,羽化率为667%。化蛹7 d后,雌、雄蛹质量分别为(037±003)g和(030±004)g;羽化7 d后,雌、雄成虫体质量分别为(027±003)g和(021±002)g,取食配方B的幼虫发育历期平均为(719±40)d,蛹期为(128±07)d,化蛹率为18%,羽化率为758%。化蛹7 d后,雌、雄蛹质量分别为(033±002)g和(027±003)g;羽化7 d后,雌、雄成虫体质量分别为(029±0.02)g和(022±003)g,取食配方C的幼虫的发育历期平均为(713±20)d,蛹期为(111±03)d,化蛹率为583%,羽化率为881%。化蛹7 d后,雌、雄蛹质量分别为(063±003)g和(049±003)g;羽化7 d后,雌、雄成虫体质量分别为(053±003)g和(043±003)g。[结论]以取食配方C饲养的天牛幼虫,其发育的各项指标均明显优于以取食配方A和B饲养的天牛。
[ Objetcive] In order to acquire large number of even-aged and consistent physiological status larvae to pro- vide enough insects for involved research, mass rearing techniques of Monoehamus alternatus were carried out on artificial diets in the laboratory. [ Method ] Developmental time and activities at different stages were compared among A, B, C three kinds of artificial diets. A (pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium ben- zoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, H2O 800 mL) , B (pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, cholesterol 1.5 g, as- corbie acid 4 g, casein 20 g, choline chloride 1 g, H20 800 mL) , C (pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12. 5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, wheat germ powder 25 g, cholesterol 1.5 g, ascorbic acid 4 g, casein 20 g, choline chloride 1 g, H20 800 mL). [ Result] When provided with di- et A, larval and pupal periods were (90.6±9.3) d and (11.9±1.3) d respectively, pupation rate was 6.4% and emer- gence rate was 66. 7%. Pupal weights were (0. 37±0.03) g for females and (0.30±0.04) g for males, and adult weights were (0.27±0. 03) g for females and (0.21±0.02) g for males after 7 days emergence. When provided with diet B, lar- val and pupal periods were (71.9±4.0) d and (12.8±0.7) d respectively, pupation rate was 18% and emergence rate was 75.8%. Pupal weights were (0.33±0.02) g for females and (0.27±0.03) g for males, and adult weights were (0.29 ±0.02) g for females and (0.22±0.03) g for males after 7 days emergence. When provided with diet C, larval and pupal periods were (71.25±2.01) d and (11.12±0.34) d respectively, pupation rate was 58.33% and emergence rate was 88. 10%. Pupal weights were (0.63±0.03) g for females and (0.49±0.03) g for males, and adult weights were (0.53±