中文摘要：

本文利用激发-发射矩阵荧光与二阶校正算法相结合，实现快速测定人体血浆样和牛蒡子对照药材中的牛蒡苷含量。采用二阶校正方法的平行因子分析（PARAFAC）和交替归一加权残差（ANWE）两种算法进行解析，所得血浆基体干扰下牛蒡苷的回收率分别为（98．7±1．4）％和（99．7±1．6）％，实现了对血浆样中的牛蒡苷含量的直接快速定量测定。此外，用上述两种方法对牛蒡子药材中的牛蒡苷含量进行快速测定，所得结果与液相色谱方法结果进行比较，结果令人满意。实验结果表明，此方法方便快捷，可用于复杂试样中未知干扰共存下牛蒡苷含量的测定。

英文摘要：

This paper proposed a new approach for direct determination of arctiin in plasma samples as well as a Chinese medicine （ Arctium lappa L. ） by the combination of excitation-emission matrix fluorescence and second-order calibration methods based on parallel factor analysis（PARAFAC） and alternating normalization-weighted error（ANWE） algorithms. The average recoveries of actiin in plasma samples obtained by using PARAFAC and ANWE with the estimated component number of two were 98.7 ± 1.4% and 99. 7 ± 1.6%, respectively. The methodology was used to estimate the content of arctiin in Arctium lappa L. , and the obtained results were also satisfactory compared with that of the HPLC method. It can be seen that the suggested methods can be emp!oyed to directly deter- mine arctiin, even in the presence of unknown and un-calibrated interferences, only with a simple pretreatment procedure.