中文摘要：

采用原子转移自由基聚合法制备了亲水聚合物修饰的硅胶颗粒作为一种新型固定化酶载体，在实现蛋白酶高密度固定的同时，显著降低了载体材料非特异性吸附导致的样品损失、因此，该固定化酶材料兼具高酶解效和高回收率的特性。以标准蛋白质牛血清白赁白（BSA）为样本，使用该固定化酶1 min即可完成酶解，鉴定到肽段对BSA的氨基酸序列役盖牢可达90％以上。该固定化酶材料成功应用于酵母菌全蛋白质复杂样本的酶解，从3min酶解产物中鉴定到666个蛋白质，超过同样条件下溶液酶解12h的鉴定结果．

英文摘要：

Abstract： A new type of immobilized trypsin was prepared using hydrophilic polymer modified silica microparticles （HPMSM） synthesized by atom transfer radical polymerization （ATRP） as the substrate material. ATRP modification led to densely packed hydrophilic polymer chains grafted on the microparticles surface which resulted in largely increased trypsin loading amount and minimized the nonspecific adsorption of proteins and peptides. Therefore, ultra-fast and highly efficient protein digestion was achieved with minimized sample loss. For standard pro- tein bovine serum albumin （ BSA）, 1 rain digestion led to the identification of 93 peptides, which covered 91% amino acid sequence of the protein. This immobilized tt~jpsin was further successfully applied to the digestion of complex protein samples from yeast and 666 proteins were identified after 3 min digestion, which exceeded the number of identified proteins after 12 1/ solution digestion.