中文摘要：

考察大豆异黄酮的微波光波组合无溶剂提取方法,发现微波光波提取干法仅用9.4%的N,N-二甲基甲酰胺DMF作为能量传递介质,功率800 W（微波55%与光波45%）加热6 min后,乙醇萃取得总黄酮。大豆苷元的提取率为0.14%,与相应的常规提取法接近,该法有操作简单快速,成本低,环境污染小等优点。利用高倍荧光显微镜FM,IR对微波提取机理进行初步研究表明,微波光波可能是从对植物组织结构的影响上来改善次生代谢产物的提取效率。

英文摘要：

Extraction of the soybean isoflavone was studied by combination method of microwave and light wave radiation without solvent.We discovered that only using 9.4% N,N-dimethyl formamide DMF as the energy transfer medium,heating the sample for 6min with power 800W（microwave 55% and light wave 45%）,the total flavanone was extracted with ethyl alcohol.The extraction rate of daidzein that was 0.14%,closed with the corresponding conventional extraction process.This method had many merits,such as simplicity of operator,rapid,low cost,small environmental pollution and so on.Extraction mechanism was investigated preliminarily with high magnification fluorescence microscope FM,IR.It indicated that the improvement of extraction efficiency of secondary metabolite was possibly because the plant tissue structure was changed by microwave.