中文摘要：

目的：利用新月弯孢霉对重楼皂苷的转化条件进行优化。方法：采用新月弯孢霉（Curvularia lunata）ATCC3.4381,利用单因素实验对其发酵条件进行优化。采用Plackett-Burman（P-B）方法筛选出对转化率有重要影响的4个因素（蔗糖、酵母膏、（NH4）2SO4和玉米浆的添加量）,并采用响应面试验设计（RSM）对重要因素进行优化。结果：最适的培养基条件为：即蔗糖浓度为7.847g/L;酵母膏的浓度为1.969g/L;硫酸铵的浓度为4.824g/L;玉米浆的浓度为11.81g/L。结论：优化后其转化能力得到了明显的提高,转化率为76.80%,并且与拟合值77.18%接近。

英文摘要：

Objective：A fermentation medium for steroid saponins biotransformation by Curvularia lunata 3.4381 had been optimized.Method：Important components and their concentrations were investigated using fractional factorial design and Plackett-Burman（P-B）.The results showed that sucrose,corn pulp,yeast extract and （NH4）2SO4 are important for biotransformation.A polynomial model related to medium components and steroid saponins yield had been established.Result：The significance of tested components was related to sucrose （7.847g/L）,yeast extract （1.969g/L）,（NH4）2SO4 （4.824g/L） and corn pulp （11.81g/L）.Conclusion：The percent of conversion reached 76.80% （p0.05）,which is close to the fitting value 77.18%.