为了更好地利用铬酸钾溶液去除UV-B光源中的UV-A和UV-C,对不同浓度和pH值的铬酸钾溶液的紫外吸收特征及其稳定性进行了相关研究。并在相同剂量UV-B作用下,对地木耳(Nostoc commune UETX-584)光合生理活性和生化组分在UV-B光源直接照射和经过铬酸钾过滤后照射的变化进行了对比研究。结果表明,铬酸钾溶液在pH值为8、0.4 mmol/L时可以有效滤除UV-B光源中的UV-C和大于340 nm的UV-A。相对于经过铬酸钾溶液过滤的UV-B光源,未过滤的UV-B光源显著抑制了地木耳的生长、叶绿素合成、Fv/Fm、最大光合作用速率和集光效率,显著促进了MAAs(三苯基咪唑类氨基酸)等吸收紫外辐射色素的合成。相对于无紫外辐射的地木耳来说,经过铬酸钾溶液过滤的UV-B对地木耳的生长影响不明显,但是其促进了紫外吸收色素的合成,抑制了Fv/Fm、最大光合作用速率和集光效率。
To determine a better way to use sodium chromate solution to remove UV-A and UV-C from UV-B light source,ultraviolet absorption characteristics of sodium chromate at different concentrations and pH levels were studied.The physiological activities and biochemical component of Nostoc commune under UV-B light source with and without filtration by sodium chromate were also studied.Results showed that sodium chromate works well to remove UV-C and UV-A at a concentration of 0.4 mmol/L and pH 8.Compared with filtered UV-B light source,the unfiltered UV-B light source inhibited the growth,chlorophyll synthesis,Fv/Fm,maximized photosynthetic rate and light harvesting efficiency of N.commune significantly.However,it also promoted the synthesis of ultraviolet absorbed material such as MAAs in the cells of N.commune.Compared with N.commune grown without UV-B radiation,N.commune grown with filtered UV-B light source had lower Fv/Fm,maximized photosynthetic rate and light harvesting efficiency but higher content of MAAs.But there was no significant difference between their growth.