中文摘要：

将栽培种花生8823体细胞胚和匍匐区组近缘野生种A．rigonii幼茎分离得到的原生质体用PEG方法融合后，培养在添加1mg／LNAA和6mg／LBAP的改良MSBs培养基中进行液体浅层培养或固液双层培养。3d后开始观察到细胞分裂，之后细胞继续分裂生长。培养7～8周后，形成直径12mm的愈伤组织。固液双层培养优于液体浅层培养，其细胞置板率高且不易聚集。

英文摘要：

Protoplasts isolated from somatic embryos of peanut cv. 8823 and slender stem of A. rigonii were fused by PEG method. The fusion products were cultured in a modified MSB5 medium containing 1mg/L NAA and 6mg/L BAP. The first cell division occurred within 3 days. Then some of the cells divided and developed into colonies. After 7 to 8 weeks, the small calli grew up to 1-2 mm in diameter.