中文摘要：

背景认知功能上的长期的应力的效果是在神经科学的热话题之一。但是在它的机制上有许多争吵。这研究的目的是在成年人老鼠随机被划分成控制和长期的多重强调的组的在 rats.Methods 的马头鱼尾的怪兽的象 Fyn， BDNF 和 TrkB 的表达式一样的空间学习和存储器上调查长期的多重应力的效果。在多重强调的组的老鼠不规则地并且或者暴露于持续 6 个星期的垂直革命，睡觉徵用和抑制的状况， 6 个小时每有为 6 个星期的夜里照明的天。在长期的多重压力的时期前后，空间学习和所有老鼠的记忆的表演用莫利斯舞水迷宫(MWM ) 被测量。在马头鱼尾的怪兽的 Fyn， BDNF 和 TrkB 蛋白质的表示是由西方的弄污和 immunohistochemical 方法的 assayed。在老鼠的马头鱼尾的怪兽的 Fyn 和 TrkB mRNAs 的层次被在控制组和强调的组的逃跑潜伏是的 RT-PCR technique.Results 检测空间学习和老鼠的存储器的 15.63 和 8.27 秒 respectively.The 性能在长期的多重强调的组(P0.05 ) 被增加。在强调的组的 Fyn， BDNF 和 TrkB 蛋白质的层次比控制组(P0.05 ) 的那些高。immunoreactivity 的结果证明 Fyn 在马头鱼尾的怪兽的 CA3 区域是在场的， BDNF 积极粒子在 CA1 和 CA3 的原子核是分布式的象 DG 小粒的房间一样的金字塔形的房间。定量分析显示 Fyn mRNA 的那水平也是在强调的组(P0.05 ) 的马头鱼尾的怪兽的 upregulated 长期的多重应力能提高空间学习和老鼠的记忆功能的 .Conclusions。Fyn， BDNF 和 TrkB 蛋白质和 Fyn mRNA 的水平的表示在 stessed 老鼠马头鱼尾的怪兽被增加。这些建议 Fyn 和 BDNF/TrkB 信号 transduction 小径可以参予在长期的多重应力期间学习提高和记忆的进程。

英文摘要：

Background The effect of chronic stress on cognitive functions has been one of the hot topics in neuroscience. But there has been much controversy over its mechanism. The aim of this study was to investigate the effects of chronic multiple stress on spatial learning and memory as well as the expression of Fyn, BDNF and TrkB in the hippocampus of rats. Methods Adult rats were randomly divided into control and chronic multiple stressed groups. Rats in the multiple stressed group were irregularly and alternatively exposed to situations of vertical revolution, sleep expropriation and restraint lasting for 6 weeks, 6 hours per day with night illumination for 6 weeks. Before and after the period of chronic multiple stresses, the performance of spatial learning and memory of all rats was measured using the Morris Water Maze （MWM）. The expression of Fyn, BDNF and TrkB proteins in the hippocampus was assayed by Western blotting and immunohistochemical methods. The levels of Fyn and TrkB mRNAs in the hippocampus of rats were detected by RT-PCR technique. Results The escape latency in the control group and the stressed group were 15.63 and 8.27 seconds respectively. The performance of spatial learning and memory of rats was increased in chronic multiple stressed group （P〈0.05）. The levels of Fyn, BDNF and TrkB proteins in the stressed group were higher than those of the control group （P〈0.05）. The results of immunoreactivity showed that Fyn was present in the CA3 region of the hippocampus and BDNF positive particles were distributed in the nuclei of CA1 and CA3 pyramidal cells as well as DG granular cells. Quantitative analysis indicated that level of Fyn mRNA was also upregulated in the hippocampus of the stressed group （P〈0.05）. Conclusions Chronic multiple stress can enhance spatial learning and memory function of rats. The expression of Fyn, BDNF and TrkB proteins and the level of Fyn mRNA are increased in the stessed rat hippocampus. These suggest that Fyn and BDNF/TrkB signal transduction p