中文摘要：

目的：研究心肌缺血和有氧运动训练诱导血管内皮细胞生长因子（VEGF）表达的时间规律。方法：成功建模的健康成年巴马小型猪24只，体重23．7±4．0kg，随机分为对照组、缺血组和运动组。将可控性水囊梗阻器安装在猪冠状动脉钝缘支处，建立可控性猪心肌缺血模型。对照组不进行缺血刺激和有氧运动训练；缺血组进行单纯缺血刺激，2min／次，2次，d，持续缺血刺激8周；运动组除进行缺血刺激外，每天进行一次平板有氧运动训练，每次30min。每两周取动脉血．采用ELISA法检测血液VEGF。实验终点时采用Western Blot检测缺血区心肌VEGF。结果：缺血组第2周血液中VEGF的表达量明显高于实验前（P〈0．05），随后进入平台期（P〉0．05）。运动组中第2—4周血液中VEGF的表达量持续增高（P〈0．05），随后达到平台期（P〉0．05）。缺血区心肌VEGF表达和血液VEGF表达呈正相关（r=0．826，P〈0．01）。结论：心肌缺血和有氧运动训练诱导的血液VEGF增高分别在第2周和第4周达到高峰，然后进入平台期。

英文摘要：

Objective：To study the temporal phenomenon of myocardial ischemia and aerobic exercises training induced expression of vascular endothelial growth factor（VEGF） in swine. Method：Twenty-four miniature swines were randomly assigned to control group （CG）, ischemia group （IG） and exercises group （EG）. A controllable hydraulic balloon catheter was placed at the first marginal branch of left circumflex coronary artery. A model of controllable myocardial ischemia was established. Subjects in IG and EG received transient coronary occlusion for 2rain repeated twice a day for 8weeks. Subjects in EG received 30 rain aerobic exercises training on treadmill with intermittent high intensity 2rain duration which repeated twice a session. Blood samples were obtained every 2 weeks. The ischemic area of the myocardium was also sampled after the completion of 8-week experiment. VEGF in serum were analyzed by ELISA and VEGF in myocardium was measured by Western Blot. Result： VEGF serum level on week 2 in IG was significantly higher than that in CG （P〈0.05） and then reached plateau （P〉0.05）. EG demonstrated the significant increase of VEGF from baseline to week 4 （P〈0.05）, and then reached the plateau （P〉 0.05）. VEGF in serum was positively correlated that in myocardium （r=0.826, P〈0.01）. Conclusion： Intermittent myocardial ischemia and aerobic exercises training induced VEGF expression reaches peak on week 2 and week 4 respectively, and then reaches plateau.